Keywords : Equine

Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi

M.A. El-Seify; N.M. Helmy; N.M. Elhawary; Sh.S. Sorour; A.M. Soliman

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 5-11
DOI: 10.33899/ijvs.2018.153787

The purpose of this study was to determine the prevalence of clinical, subclinical and chronic infection with the equine parasite T. equi in some Egyptian localities (Cairo and Giza governorates). A panel of 396 equine blood samples representing 141 horses, 250 donkeys and 5 mules was collected from equines during the period from April 2015 to March 2016 using microscopic examination and conventional PCR. Microscopically a twenty two (5.56%) of 396 were positive for T. equi merozoites that appeared as small rounded, pyriform shaped and maltase cross shaped merozoites. Among 8/141(5.67%) horses and 14/250 (5.60%) donkeys were found to have positive for T.equi. A one hundred blood samples (45 horses, 50 donkeys and 5 mules) selected randomly were also examined by PCR. The results of PCR showed 30/100(11/45 (24.4%) horses, 18/50 (36%) donkeys and 1/5 (20%) mule) were positive for T.equi. When the sequenced PCR amplicons (n=3) were aligned to the reference nucleotide sequences of T. equi accessed in Genbank, the horse isolate showed insertion of Thymine (T) base at position 23 and substitution of Thymine (T) base with Cytosine (C) base at position 91, while the donkey and mule isolates have no alterations when compared to the reference sequences. The phylogenetic analysis showed that the sequenced PCR isolates belonged to T. equi. The obtained sequences were deposited in the GeneBank database under accession numbers MF192854, MF192855 and MF192856.

Detection of Toxoplasma gondii antibodies in horses in Mosul, Iraq

Mohammad N. Alshahery; Reem S. Mansour

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 39-41
DOI: 10.33899/ijvs.2012.168677

This study was aimed to verify the presence of Toxoplasma gondii antibodies in equine sera in Mosul city, Iraq. Seventy nine samples of sera were examined (70 female and 9 male) by latex agglutination test (LAT) and 2–Mercaptoethanol test (2-ME). Results showed that anti-bodies to T. gondii using LAT were detected in 72.2% (71.4% female and 77.8% male) whereas 57% (57.1% female and 55.6% male) of infected horses were detected by 2-ME. 

Detection of microfilaria infection in horses in Mosul city

E. G. Suleiman; S. S. Aghwan; O. M. Al- Iraqi

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 23-26
DOI: 10.33899/ijvs.2020.167131

The study was performed on 78 horses in Mosul city were examined from April 2007 to September 2009 for the detection of microfilaria in the blood Twenty five clinically normal animals served as control. The percentage of the infection with microfilaria of Setaria spp was 30.76% they were sheathed, measuring 204-228 µm within a mean of 218.4 µm in length and 4.5-4.8 mµ within a mean of 4.62 µm in width. A high percentage of infection was reported in females 54.28%) with significant differences from that of males. No significant differences were noticed between the healthy and these showing some clinical sings or between these treated with ivermectin or non treated. Haematological changes indicated decrease in level of hemoglobin concentration packed cell volume whereas there was significant increase (P≤0.05) in total leukocytes count, anemia was macrocytic hypochromic type.