Keywords : Ovaries

Ovario-utero protective effect of silymarin in ethidium bromide treated female rats

Hayder G. Abdulshaheed; Jabber A. Al-Saaidi; Jawad K. Al-Arak

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 1, Pages 213-211
DOI: 10.33899/ijvs.2021.129798.1688

This study was conducted to qualify the ameliorating potency of silymarin against toxicity in ethidium bromide (EtBr) treated female rats. Eighty female Wistar rats aged 100 days, weighted 170-175 g were randomly allocated to control, orally supplemented with drinking water, and three treated groups, orally supplemented with silymarin 200 mg/kg BW, EtBr 10 mg/kg BW, and combination of EtBr and silymarin (SEtBr), respectively. Each group was allocated to two subgroups, sacrificed after 20 and 40 days of treatment. Bodyweight gain, uteri, and ovaries weight were recorded. Ovarian samples were obtained for histopathological examination. EtBr group females recorded the lowest body weight gain, relative weights of ovaries and uteri, and ovarian follicle number, whereas S group females recorded the highest body weight gain and follicular number, while the ovaries and uteri weights were either higher or close to the control group, at both experimental periods. Histopathological findings of both periods revealed necrosis, cirrhosis, ischemia, and prominent hemorrhage in the blood capillaries in EtBr treated ovarian tissues, but many of the ovarian follicles being mature and the atretic follicles were hence found to be high in number, whereas silymarin treated females showed normal ovarian tissues and viable ovarian follicles as that in control females. The combination-treated females, at 20 days, revealed necrotic primary ovarian follicles with some macrophages infiltration, whereas 40 days’ period showed normal ovarian cortex, medulla, and ovarian follicles. In conclusion, silymarin treatment in combination with EtBr has a potent amelioration effect against ovarian toxicity, in a duration-dependent manner.

Histopathological alterations of male and female reproductive systems induced by alloxan in rats

Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 2, Pages 223-226
DOI: 10.33899/ijvs.2020.126626.1351

The objective of this study is to determine the histological effects experimentally induced by injection of alloxan 100 mg/kg B.w. on the histopathological structure of reproductive organs of male and female albino rats. The results showed that treatment with alloxan cause alteration in testis include irregular shape and size of seminiferous tubules, irregular division of spermatid cells, degeneration and necrosis of Sertoli cells and paucity of sperms in the lumen of tubules. While histological examination of epididymis showed the lumen of it free from sperms, thickening of muscular layer and interstitial tissue between the epididymis canal. The histological alteration of female reproductive organs includes disturbances in development of primary follicles of ovaries, hemorrhage in the interstitial tissue as well as atrophy in the uterine glands with hyperplasia of the epithelial cells of uterus. The conclusion of this study showed that alloxan cause histological alteration in reproductive organs of male and female rats.

Effects of red reishi mushroom (Ganoderma lucidum) on the reproductive system in female and male rats

Buthina Abdulhameed Abdullah; Maad Alfahad; Dakhell Hussein Hdree

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 137-141
DOI: 10.33899/ijvs.2019.125547.1067

The present study aimed to investigate the histological changes of Ganoderma lucidum (Red Reishi mushroom) on female and male reproductive system by using light microscope. Fifteen white rat weighing 200-250g was used in the experiments. The animals was divided mainly into three groups and these groups were subdivided into 3 groups for female, and 3 groups for male, that’s group represented as control group without treatment (G1). The 2nd received only 0.03 gm and vitamin C diluted with D.W. and the 3rd group received 0.03 gm of Ganoderma lucidum . The results of the present study showed that there are no side efects of Ganoderma lucidum on female and male genital system of rats (positive effects).