Keywords : Embryos

Histopathological and Some Biochemical Effects of Platinum Drug on the Liver and Kidney of Pregnant Mice Mus musculus and their Embryos

Baidaa A. barwarei; Hanan S. Sadoon

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126793.1382

The current study was done to investigate the effect of two doses 3, 6 mg/kg b.w. of Platinum drug on the structure of the liver and kidneys of pregnant mice and their embryos and weight changes including ; mothers body weight, embryos,maternal liver and kidneys, as well as some serum biochemical parameters were established. Thirty pregnant mice were used and they were divided into three groups (10 mice/group),as the following ; group I (control group); animals were injected intraperitonialy (IP) with distilled water on the days 7th,12th,and 17th of gestation,whilst groups II,III were injected intraperitonialy (IP) with the selected doses above of platinum on the days 7th, 12th, 17th of gestation,respectively. Microscopically; maternal and fetal' liver sections of group II revealed; vaculation, swelling, apoptosis and infiltration of inflammatory cells, congestion, degeneration and involvement of extra medullary hematopoietic cells, respectively. Pervious lesions were increased in both maternal and fetal’ liver of group III. Maternal and fetal kidney sections of group II revealed ; degeneration, expansion of Bowman's space, inflammatory cells infiltration into interstitial tissue, and blood capillary congestion The previous lesions showed more severity in group III.

Pathological study of intrauterine infection to embryos by Encephalitozoon cuniculi spores in pregnant mice

Saevan Saad Al-Mahmood; H.I. Al-Sadi

Iraqi Journal of Veterinary Sciences, 2016, Volume 30, Issue 2, Pages 49-54
DOI: 10.33899/ijvs.2016.121383

This study aimed to investigate pathology of oral E. cuniculi infection during pregnancy in pregnant mice and embryos. A total of 40 pregnant mice at first day of gestation were divided into two groups, first group were infected orally by E. cuniculi sores of 107 spores/ mice, second group left without any treatment. At 18th days of gestation all pregnant mice were euthanized. Gross pathology finding in pregnant mice of infected group included congestion of liver and lung, the embryos lesions consisted from enlargement of head and abdomen. Histological lesions in pregnant mice of infected group consisted of hepatic non-suppurative granulomatous lesions with E. cuniculi spores aggregation with lymphocytic infiltration, the lungs lesions consisted of infiltration of lymphocytes with E. cuniculi spores, kidney lesions composed from degenerative and necrotic changes in renal tubules, brain lesions consisted from lymphocytic infiltration with increase in number of glial cells, while intestine tissue sections showed hyperplasia of lymphatic tissue with present of parasitic vacuoles at tips of villi, the placenta exhibited E. cuniculi spores with hyperplasia of trophoblast in chorionic villi, while histological lesions in embryos showed lymphocytic infiltration around alveoli with hyperplasia of lymphatic tissue around bronchioles with absent the normal architecture of hepatic cords and vacuolation of hepatocytes with hyperplasia of lymphocytes in white pulp of spleen. This study provides insight into the pathology of E. cuniculi infection in pregnant mice and their embryos, also supports the hypothesis of intrauterine transmission of E. cuniculi infection to embryos during pregnancy period.