Keywords : Brucellosis

A cross sectional study on the seroprevalence of bovine brucellosis in Al-Najaf province in Iraq

Khaleel Z. Al-Alo; Ahmed J. Mohammed

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 617-620
DOI: 10.33899/ijvs.2021.127143.1471

Brucellosis is an endemic disease in many countries of the middle east including Iraq, despite the high attempts to control the disease in animals using vaccination programs. The study was conducted from January - March 2012, to determine the prevalence of bovine brucellosis in the Al-Najaf province in Iraq, which represent risk factors associated with brucellosis in cattle. Blood samples were collected from 172 cows and 15 bulls of 20 dual-purpose cattle herds. Serological examinations are the most useful and widely used tool for the diagnosis of brucellosis in man and animals. All animal serum samples were tested by the Rose Bengal plate test (RBT), and the positive cases were confirmed by competitive enzyme-linked immunosorbent assay (c-ELISA). The overall prevalence of brucellosis was 5.81% divided between 5.81% in females and 0% in males, and 40% as herd numbers level. Females showed a higher seropositive reaction to the disease than males. In conclusion, this study demonstrated the presence of the disease and detected the seropositive reactions in animal samples in different farms of the province, with a difference in infection rate regarding the risk factor. Sanitary measures must be taken to avoid the occurrence and spread of the disease in humans and animals.

Serological and molecular investigations of Brucellosis in dairy cows at certain areas of Al-Sulaymaniyah governorate- Iraq

Kani M. Ridhae; Suha A. Hussein

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 657-662
DOI: 10.33899/ijvs.2021.127688.1520

This study aimed to detect Brucella antibodies in the sera of dairy cows and to identify Brucella species in the milk of seropositive cows. A total of 100 sera and 100 milk samples were collected from two 50-cows groups (group 1 with and group 2 without a history of reproductive problems and/or decreased milk production). Rose Bengal plate test and indirect ELISA were used to explore Brucella antibodies in the serum samples and thereafter milk samples of seropositive cows were undergone PCR analysis using Brucella genus specific primers and 3 pairs of species specific primers for identification of B. abortus, B. melitensis and B. suis. The RBPT showed 22 cows were carriers for the Brucella antibodies, 18 in group 1 and 4 in group 2 whereas the iELISA showed only 10 cows out of these 22 cows were positive, 9 in group 1 and only 1 cow in group 2. The PCR assay, which was performed on milk samples of the RBPT positive cows, revealed 18 samples were positive for the Brucella genus and the Brucella abortus species and were negative for Brucella melitensis and Brucella suis species. As a conclusion, the results of this study showed that brucellosis has been encountered in cows with or without a history of reproductive problems, and the RBPT followed by PCR assay for milk samples of the seropositive cows could provide more specific detection than performing either test alone and could be more useful for rapid screening of brucellosis in dairy cows.