Keywords : rats
Evaluation of the thermal effect of LTE 2600 MHz (4G) electromagnetic field (EMF) exposure: Thermographic study on rats
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 2, Pages 279-285
DOI:
10.33899/ijvs.2020.126787.1379
Exposure to LTE 2600 MHz microwaves is increasing very fast as new technologies and become accessible worldwide, and the smartphones being the main source of these waves. The aim of this study is to assess the thermal effect of 4G signals on rats. Forty adult Albino rats were used throughout the study, assigned as control and exposed groups, equally. Rats were kept in Plexiglas cages with intermittent exposure to LTE mobile-phone like signals at an average of 2h/day for up to 30 continuous days with SAR value of 0.982 W/kg. Infrared images were snapped immediately after the end of the exposure time, then one hour, two hours, and four hours later at a rate one collection/week during the study. IR images were analyzed by FLIR Tools software. The results exhibited variation in reflected skin temperatures in the exposed group compared to control images. Furthermore, the analysis of collected data revealed significant variations over the course of the study compared to the first week. The rise in skin temperature observed in response to exposure in the first week, which decreased gradually increased exposure and this drop in reflected skin temperature was significantly related to amount of exposure. The study concludes that the LTE 2600 MHz exposure under controlled laboratory conditions has a thermal effect on the rats.
A histological study on the effect of imatinib on the rats' testis after early postnatal exposure
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 85-92
DOI:
10.33899/ijvs.2020.126342.1303
The safety region of imatinib, and markedly its role in testicular toxicity has been studied in a controversial manner in current years. This study was designed to address the repercussion of administration of groups of pups with imatinib mesylate (at neonatal or infantile periods) using histological analysis of their testes in several end points and in adulthood. Intact pups (albino-male) exposed to 200 mg/kg of oral imatinib once daily at neonatal and early infantile period on PND 1 to PND10 (for ten days). All experiments performed with age and weight matched control which administered with Distilled water. Pups were into categorized into 4 subgroups, according to the ages of euthanasia: 15 days postpartum (PND); 40days postpartum; 70 days postpartum and 140 days postpartum. The histological analysis was conducted in blind fashion after staining with (Harris Hematoxylin and Eosin stain. Ten randomly selected testicular sections from each rat were analyzed qualitatively and quantitavely. In addition, Johnsen' scores were used to analyze the effect of drug on spermatogenesis. Data were recorded and value is considered as significant when it P
Toxic effects of butylated hydroxytoluene in rats
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 121-128
DOI:
10.33899/ijvs.2020.126435.1322
This study aimed to assess the acute toxicity in rats of heated and un heatedbutylated hydroxytoluene (BHT). Sunflower oil dissolved BHT, heated at 98±2ºC by a water bath. The animals were divided into five groups. The control group dosage orally with sunflower oil, the first group treated with 250 mg/kg BHT, the second group treated with 250 mg/kg heated, BHT the third group treated with 500 mg/kg BHT and the fourth group treated with 500 mg/kg heated BHT. All groups received oral treatment. The results showed a substantial reduction in motor activity relative to other groups at a dose of 250 mg/kg heated BHT. There was a substantial distinction in the negative geotaxis test in groups of 500 mg/kg heated and un-heated BHT, while a cliff avoidance test in the heat treated dose of 250 and 500 mg/kg was observed in the cliff avoidance test compared to other groups. A significant reduction occurred in all groups in the pocketing and dorsal tonic immobility test. The pathological changes of heated BHT groups were more severe than those of un-heated BHT groups especially the dose of 500 mg/kg heated BHT. It represented by coagulative necrosis, muscle atrophy in heart, interstitial pneumonia, serofibrinous exudate, pulmonary emphysema in lung and neuronal degeneration, microgliosis, myelin vacuolation and satellitosis in the brain. The study concluded that heated BHT at a dose of 250 and 500 mg/kg had toxic effects to motor and neurobehavioral activity, and histopathological changes in the brain, heart, and lung.
Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 151-156
DOI:
10.33899/ijvs.2020.126490.1335
This study aims to investigate the effect of aspirin in methotrexate toxicity (increase or decrease) relating to oxidative stress and histopathological changes of the liver and kidney in male rats. Twenty rats were divided into equal 4 groups, the first is considered control group, the second was treated with methotrexate in a dose of 10 mg/kg, the third was treated with methotrexate and aspirin in doses of 10 and 30 mg/kg respectively, the fourth was treated with aspirin alone with a dose of 30 mg/kg. All doses were given by daily oral dosage for 4 constitutive days. The result revealed a significant decrease in the concentration of both glutathione and albumin and a significant increase in the concentration of both malondialdehyde and ALT enzyme in the two groups treated with methotrexate alone or in combination with aspirin as compared to the control group. The histopathology revealed that the severity of lesions was in the group of methotrexate with aspirin, group of methotrexate only and a group of aspirin respectively, which are representing by coagulative necrosis and hypertrophy of hepatocytes in the liver while the lesions of kidney were atrophy of some glomeruli and renal cystic formation. The study concludes that aspirin increases the toxic effect of methotrexate at the level of oxidative stress concomitant with the occurrence of hepatic and renal toxicity.
The relationship between HSP70 and level of leptin and luteinizing hormones in female rats exposed to chronic and acute heat stress
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 37-43
DOI:
10.33899/ijvs.2019.125565.1082

Some anti-diabetic properties of Prosopis farcta extracts in alloxan induced diabetic in adult rats
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 109-113
DOI:
10.33899/ijvs.2019.125557.1076

Protective effects of coenzyme Q10 against sodium fluoride-induced reproductive disorders in male rats
Iraqi Journal of Veterinary Sciences,
2019, Volume 33, Issue 1, Pages 143-149
DOI:
10.33899/ijvs.2019.125534.1056
This study was conducted to investigate the possible protective role of (CoQ10) on reproductive dysfunction of male rats induced by sodium fluoride (NaF). thirty two rats were divided into four even groups and treated for 56 days as follows: the first group served as control (C) The second group (G1) received coenzyme Q10 at dose of 10 mg/Kg.B.W; while third group (G2) received 100ppmsodium fluoride in drinking water and fourth groups (G3) received NaF 100 ppm and CoQ10 10 mg/Kg.B.W for 56 days. The results showed that exposure of rats to NaF caused decrease in serum testosterone (T) and luteinizing hormones (LH) concentrations. Whereas Administration of CoQ10 caused an increase the concentrations of theses hormones in group G3 as compared with group G2. Testicular morphometric and histopathological alterations were observed in group G2 in the form of marked microvacuolation in the basal level of germinal cells, amyloid deposited within seminiferous tubules with disarrangement and depletion of germ cells. Whereas testicular histological alterations were improved in rats treated with CoQ10. In conclusion, the results of the present study showed that use of Co Q10 can alleviating the deleterious effects on male reproductive function following exposure to NaF, may be via improvement the testicular functions or other related endocrine glands.
Effects of cadmium chloride on some endocrine glands (thyroid and adrenal) in male rats (Rattus norvegicus)
Iraqi Journal of Veterinary Sciences,
2018, Volume 32, Issue 2, Pages 211-217
DOI:
10.33899/ijvs.2019.153852
This study was designed to evaluate the cytotoxic effects of cadmium chloride (CdCl2) on thyroid and adrenal gland of male rats (Rattus norvegicus). Thirty six male rats were used and randomly into three groups each of 12 rats, the first group(G1) kept as at control. The 2nd and 3rd groups (G2 and G3) were administrated orally cdcl2 at doses of 15 and 20 mg/kg.B.W. respectively for 6 weeks. After the treatment period, the rats were sacrificed, then thyroid and adrenal glands were removed and processed for light microscope. Light microscopic examination of thyroid gland with (15mg/kg.B.W.) showed increase in the size of follicles, some of these follicles appeared empty from any colloidal substance and heavily infiltrated by inflammatory cells, with depletion of parafollicular cells. Vaculated follicles may be clear in other sections, and showed congested blood vessels, vascularized stroma, hyperplasia of thyrocyte in rats treated with 20 mg/kg.B.w. Results also noted changes in different regions of adrenal gland related to rats exposure tocdcl2 with 15 mg/kg.B.W., these includes cellular debris, necrosis of cortical cells, amorphous material in the zona fasciculate and focal necrosis in zona granulosa with disruption of normal structure and replaced by necrotic cells and inflammatory cells, also obvious adipocytes infiltration which appeared clear in medulla. Most changes occurred within the adrenal cortex and appeared more severity than that in the medulla region. Hypereosinophilia, pyknotic nuclei, congested blood vessels, hemorrhage, degeneration and inflammation also seen in both regions (cortex and medulla) of adrenal gland from rat exposed to cdcl2 at 20 mg/kg.B.W.