Keywords : Rats


Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

Ihsan Husain Mohammed Ali; Qasim Hasso Abdullah; Omer Abdul Majeed AL-Habib

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126455.1333

This study aims to investigate the effect of aspirin in methotrexate toxicity (increase or decrease) relating to oxidative stress and histopathological changes of the liver and kidney in male rats. Twenty rats were divided into equal 4 groups, the first is considered control group, the second was treated with methotrexate in a dose of 10 mg/kg, the third was treated with methotrexate and aspirin in doses of 10 and 30 mg/kg respectively, the fourth was treated with aspirin alone with a dose of 30 mg/kg. All doses were given by daily oral dosage for 4 constitutive days. The result revealed a significant decrease in the concentration of both glutathione and albumin and a significant increase in the concentration of both malondialdehyde and ALT enzyme in the two groups treated with methotrexate alone or in combination with aspirin as compared to the control group. The histopathology revealed that the severity of lesions was in the group of methotrexate with aspirin, group of methotrexate only and a group of aspirin respectively, which are representing by coagulative necrosis and hypertrophy of hepatocytes in the liver while the lesions of kidney were atrophy of some glomeruli and renal cystic formation. The study concludes that aspirin increases the toxic effect of methotrexate at the level of oxidative stress concomitant with the occurrence of hepatic and renal toxicity.

Toxic effects of butylated hydroxytoluene in rats

Yamama Z. Alabdaly; Entisar Kh. Al-Hamdany; Entisar R. Abed

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126435.1322

This study aimed to assess the acute toxicity in rats of heated and un heatedbutylated hydroxytoluene (BHT). Sunflower oil dissolved BHT, heated at 98±2ºC by a water bath. The animals were divided into five groups. The control group dosage orally with sunflower oil, the first group treated with 250 mg/kg BHT, the second group treated with 250 mg/kg heated, BHT the third group treated with 500 mg/kg BHT and the fourth group treated with 500 mg/kg heated BHT. All groups received oral treatment. The results showed a substantial reduction in motor activity relative to other groups at a dose of 250 mg/kg heated BHT. There was a substantial distinction in the negative geotaxis test in groups of 500 mg/kg heated and un-heated BHT, while a cliff avoidance test in the heat treated dose of 250 and 500 mg/kg was observed in the cliff avoidance test compared to other groups. A significant reduction occurred in all groups in the pocketing and dorsal tonic immobility test. The pathological changes of heated BHT groups were more severe than those of un-heated BHT groups especially the dose of 500 mg/kg heated BHT. It represented by coagulative necrosis, muscle atrophy in heart, interstitial pneumonia, serofibrinous exudate, pulmonary emphysema in lung and neuronal degeneration, microgliosis, myelin vacuolation and satellitosis in the brain. The study concluded that heated BHT at a dose of 250 and 500 mg/kg had toxic effects to motor and neurobehavioral activity, and histopathological changes in the brain, heart, and lung.

Some anti-diabetic properties of Prosopis farcta extracts in alloxan induced diabetic in adult rats

Ismael H. Mohammed; Ismail S. Kakey; Mahdi M. Farimani

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 109-113
DOI: 10.33899/ijvs.2019.125557.1076

Prosopis farctais belonged to Mimosaceae family, commonly known as mesquite. It was chosen to investigate their effect on α-glucosidase inhibitory activity (in-vitro), serum glucose and liver functions. Roots, fruits, and leaves of P. farcta, were extracted by n-hexane, ethyl acetate, and methanol. α-glucosidase inhibition was analyzed by using ELISA technique then half maximal inhibitory concentration IC50 was found. The blood glucose levels were determined with a glucose analyzer model. The serum alkaline phosphatase (ALP), Alanine transaminase (ALT), aspartate aminotransferase (AST) and serum total bilirubin (TB) were estimated by using the Cobas diagnostic kit with a fully automated chemical analyzer. Diabetes was done by a single dose of 120 mg alloxan/kg b.w with subcutaneously injection. Ethyl acetate extracts of P. farcta showed the higher α-glucosidase inhibitory activity, and the best one was root extract. Treatments of the alloxan-induced diabetes rats were done by daily oral administration of different concentrations with P. farcta extracts of ethyl acetate for 28 days and the dose 200 mg/kg BW was the effective one. The root extract was the best one for reduction of serum glucose followed by leaves then fruits. Administration of root extract of P. farcta showed a decrease in the levels of ALP and TB in alloxan-induced diabetes rats. The fruit extract of P. farcta showed decreasing in the level of ALP in alloxan-induced diabetes rats. In conclusion, the P. farcta extracts for ethyl acetate have properties of hypoglycemic effect as well as improving some parameters related with diabetic complications of liver functions.

The relationship between HSP70 and level of leptin and luteinizing hormones in female rats exposed to chronic and acute heat stress

Hiyam N. Matty; Ashwaq A. Hassan

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 37-43
DOI: 10.33899/ijvs.2019.125565.1082

The current search experiments designed to study the effect of chronic and acute heat stress on the relationship between HSP70, leptin and luteinizing hormones level in female rats. 45 female pups were used in the age of weaning, were randomly divided into three equal groups. 1st group was a control group, 2nd group exposed to chronic heat stress at 38 ºC for 1hour a day since the age of weaning until onset of puberty and the 3rd group exposed to acute heat stress at 38 ºC for 4 hours per day for 5 consecutive days from 35 day age of rat and each group above were secondary divided into 3 age groups consisted of pre-puberty, at puberty and post-puberty. Results of study showed female rats exposed to chronic and acute stress led to significant increase in the level of luteinizing hormone at onset of the puberty. The result showed correlation coefficient between the level of HSP70 and leptin hormone significantly in the females acute stressed group at onset of puberty, as well as female exposed to chronic heat stress led to significant correlation coefficient between HSP70 and leptin hormone at post puberty. The result revealed significant correlation coefficient between HSP70 and luteinizing hormone in female control group at onset of puberty. It concluded from this study there is relationship between HSP70 and leptin hormone at onset puberty during acute heat stress and same relationship at post puberty during chronic heat stress.

Protective effects of coenzyme Q10 against sodium fluoride-induced reproductive disorders in male rats

Sabreen M. Momammed Ali; Ahmed Jasim Nowfal; Bara Najim Abdillah

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 143-149
DOI: 10.33899/ijvs.2019.125534.1056

This study was conducted to investigate the possible protective role of (CoQ10) on reproductive dysfunction of male rats induced by sodium fluoride (NaF). thirty two rats were divided into four even groups and treated for 56 days as follows: the first group served as control (C) The second group (G1) received coenzyme Q10 at dose of 10 mg/Kg.B.W; while third group (G2) received 100ppmsodium fluoride in drinking water and fourth groups (G3) received NaF 100 ppm and CoQ10 10 mg/Kg.B.W for 56 days. The results showed that exposure of rats to NaF caused decrease in serum testosterone (T) and luteinizing hormones (LH) concentrations. Whereas Administration of CoQ10 caused an increase the concentrations of theses hormones in group G3 as compared with group G2. Testicular morphometric and histopathological alterations were observed in group G2 in the form of marked microvacuolation in the basal level of germinal cells, amyloid deposited within seminiferous tubules with disarrangement and depletion of germ cells. Whereas testicular histological alterations were improved in rats treated with CoQ10. In conclusion, the results of the present study showed that use of Co Q10 can alleviating the deleterious effects on male reproductive function following exposure to NaF, may be via improvement the testicular functions or other related endocrine glands.

Effects of cadmium chloride on some endocrine glands (thyroid and adrenal) in male rats (Rattus norvegicus)

K. H. Al- Derawi

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 211-217
DOI: 10.33899/ijvs.2019.153852

This study was designed to evaluate the cytotoxic effects of cadmium chloride (CdCl2) on thyroid and adrenal gland of male rats (Rattus norvegicus). Thirty six male rats were used and randomly into three groups each of 12 rats, the first group(G1) kept as at control. The 2nd and 3rd groups (G2 and G3) were administrated orally cdcl2 at doses of 15 and 20 mg/kg.B.W. respectively for 6 weeks. After the treatment period, the rats were sacrificed, then thyroid and adrenal glands were removed and processed for light microscope. Light microscopic examination of thyroid gland with (15mg/kg.B.W.) showed increase in the size of follicles, some of these follicles appeared empty from any colloidal substance and heavily infiltrated by inflammatory cells, with depletion of parafollicular cells. Vaculated follicles may be clear in other sections, and showed congested blood vessels, vascularized stroma, hyperplasia of thyrocyte in rats treated with 20 mg/kg.B.w. Results also noted changes in different regions of adrenal gland related to rats exposure tocdcl2 with 15 mg/kg.B.W., these includes cellular debris, necrosis of cortical cells, amorphous material in the zona fasciculate and focal necrosis in zona granulosa with disruption of normal structure and replaced by necrotic cells and inflammatory cells, also obvious adipocytes infiltration which appeared clear in medulla. Most changes occurred within the adrenal cortex and appeared more severity than that in the medulla region. Hypereosinophilia, pyknotic nuclei, congested blood vessels, hemorrhage, degeneration and inflammation also seen in both regions (cortex and medulla) of adrenal gland from rat exposed to cdcl2 at 20 mg/kg.B.W.