Keywords : Pathological

Pathological and histochemical study on cats and mice experimentally infected with TOXOPLASMA GONDII

E. R. AL-Kennany; A. M. A. Al-Saidya

Iraqi Journal of Veterinary Sciences, 2006, Volume 20, Issue 2, Pages 249-263
DOI: 10.33899/ijvs.2006.45821

In this Study, Toxoplasma gondii was diagnosed and isolated successfully from domestic (Stray) cats at Mosul city, Iraq using Latex test and the flotation method of fecal material. The study also included experimental induction of the condition by giving orally oocysts of T. gondii to albino mice at a dose of 1000 sporulated oocysts and leaving the mice for 14 days. All infected mice (15 with tissue cysts) were fed to 8 cats negative for latex and fecal flotation method. The pathogenesis of the isolated T. gondii has been followed through daily fecal examination which indicated the presence of oocysts at the 5 th post infection
(p. i.) day. Postmortem examination of 2 cats was done at 3, 7, 14, and 21
days p. i. with preparation of histological section from the intestines, liver, spleen, pancreas and brain. Additionally, histological techniques were done for the intestines and liver. Clinical sings were not seen in cats infected experimentally. Grossly, multifocal areas of necrosis and severe congestion were seen most prominently in the brain. Histiochemically, there was an increase in mucopolysaccharide in the intestine and in glycogen infiltration in hepatic cells.

Pathological study of toxoplasma gondii expermintally infected in broiler chicken

O. S. Beyon; E. S. AL-Alaf; E. R. AL-Kennany

Iraqi Journal of Veterinary Sciences, 2006, Volume 20, Issue 2, Pages 241-248
DOI: 10.33899/ijvs.2006.45818

This research was conducted to explore the pathological changes produced from the infection with oocyst of Toxoplasma gondii isolated from infected cats in broiler chicken. The research showed histopathological lesions in liver, intestine, spleen, brain and lungs.
Histopathological examinations of liver revealed perivascular tachyzote cuffing and localization of tissue cyst in hepatocyte, and intestine showed hyperplasia with presence of tachyzoite in epithelial lining of villi and submucosal glands, while in spleen localization of tissue cyst in arteriosclerosis have been seen, moreover, in brain there was parasitemia associated with penetration of tachyzoite in red blood cells have been seen and in the lung, showed proliferation of tachyzoite in epithelial lining alveoli associated with infiltration of mononuclear inflammatory cells.