Keywords : liver


Effect of levofloxacin on some body tissues in mice

Rand A. Abdullah; Faten D. Taee; Imad A. Thanoon

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126416.1316

Levofloxacin is a third generation of fluoroquinolones family. It is commonly used in the treatment of a several bacterial infections. However, misusing antibiotics impose damage to hepatocytes and myocytes. The present study was conducted to access the effect of levofloxacin on tissue histology in mice taking in consideration dose and duration of exposure. 24 matured male Albino mice were divided into 3 groups, Group G1: was considered as a control group. They received normal saline intraperitoneally / day. Group G2: They received 10.7 mg/kg/day of levofloxacin intraperitoneally for 10 days. Group G3: They received 10.7 mg/kg/day of levofloxacin intraperitoneally for 3 weeks. Microscopic examination of liver sections of group G2 revealed severe congestion of blood vessels in the portal area and central veins with inflammatory cells infiltration. While in group G3, Apoptosis, Degeneration and necrosis of hepatocytes with giant cell transformation were noticed. In addition to kupffer’s cell activation. Heart sections showed moderate congestion of blood vessels with edema in between the myocytes and inflammatory cells infiltration. Group G3 Necrosis with pyknosis of cardiac muscle nuclei was noticed. We concluded that levofloxacin induces toxic effects on liver and heart according to the dose of administration and duration of treatment.

Fasciolosis: grading the histopathological lesions in naturally infected bovine liver in Mosul city

Saevan Saad Al-Mahmood; Hadil Basim Al-Sabaawy

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 379-387
DOI: 10.33899/ijvs.2019.125546.1066

Fasciolosis cause economic losses in cattle that breed in Iraq and the world. About 4% of bovine liver’s samples included in the current study exhibited classical pathological lesions of fasciolosis. Samples of cattle livers infected with fasciolosis were taken for histopathology. Eighteen grading criteria with four scoring level have been chosen to grading the microscopic lesions caused by Fasciola hepatica into a mild infection (grade I), moderate infection (grade II) and severe infection (grade III). The type of hepatic degeneration or necrosis, cloudy cell swelling, coagulative necrosis, infiltration of inflammatory cells, with patterns of infiltration, also type of infiltrated cells, fibrosis between hepatic cells or in portal area, affection to hepatic cords arrangement, hepatic sinusoids, extensions of hemorrhage, pigment deposition, hyperplasia of bile duct, thickness of hepatic capsule and presence of liver fluke were the main grading levels. In grade, I the microscopic lesions were characterized by simple or mild in their nature with very good reversible prognosis, while grade II characterized by moderate severity of the lesions with a good reversible prognosis, while grade III characterized by hostile severity with bad irreversible prognosis as a result of architecture changes in liver histology. In conclusion, we believed that this grading system could be used as a guide when examining histopathological liver's samples infected with F. hepatica to identify the stage of infection and proposed an accurate prognosis.

Biochemical and histopathological study of thioredoxin reductase isolation from blood serum in normal and oxidative stress-exposed rats

A.A. Hamdon; L.A. Al-Helaly

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 115-124
DOI: 10.33899/ijvs.2019.163243

The study included investigation of effects of the thioredoxin reductase isolated from serum of human on oxidative stress induced in rats, through histopathological examination of the heart and liver, and the measurement of the biochemical parameters, which included: thioredoxin reductase, creatine kinase, alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin and uric acid. Treating experimental animals with 1% hydrogen peroxide led to a significant increase in thioredoxin reductase, creatine kinase, aspartate aminotransferase and total bilirubin compared to control group, while a significant decrease in: albumin and uric acid, but non-significant in alanine aminotransferase. As well as, a different dose 2 and 4mgl Kg of rat body weight of isolated TrxR with 1% hydrogen peroxide improved parameter levels through decrease oxidative stress that induced in rats. The results of the histopathological examination revealed slight to moderate changes in the heart, while no distinguishing changes were observed in the heart of the group treated with hydrogen peroxide and injected with enzyme in intraperitoneally with 4 mg/ kg of rat weight compared to control group. In the liver, there was observed vascular degeneration and thickening of hepatic capsule as a result of chronic congestion and degeneration in the blood vessels which after treatment with 1% hydrogen peroxide compared to control group, but there was noticeable improvement in the liver of group treated with hydrogen peroxide and injected with the enzyme in intraperitoneally with 4 mg/kg of rat weight, and these results confirm the role of the enzyme in the protection of the body from oxidative stress, the use of the enzyme can reduce the severity of different diseases.

Pathological study of subchronic oral toxicity induced by Di (2- ethyl hexyl) phthalate in Japanese quails

Saevan Saad Al-Mahmood; Saeed Majed Al-Abady

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 67-71
DOI: 10.33899/ijvs.2017.145567

In present study, we aimed to investigate the pathological changes induced by subchronic oral toxicity induced by sub lethal dose of Di (2-ethyl hexyl) phthalate (DEHP) in Japanese common quail. Forty male quails at one day old were divided randomly into two equal groups, the first group (treated group) was orally administrated by 10% of LD50 of DEHP (557.41 mg/kg of body weight) for 42 days, while second group (control group) left without treatment throughout experiment period. The results of current study showed that there is congestion of kidney, microscopically they showed presence of hyaline degeneration in renal tubules, glomerulosclerosis, wide spread of degenerative and necrotic lesions in renal tubules, hemorrhage, infiltration of inflammatory cell especially macrophages and lymphocytes in interstitial tissue of kidney. While grossing of liver showed hepatomegaly with hemorrhage, microscopically the sections showed wide distributing of vacuolar degeneration (fatty change) in different size and shape of vacuoles in cytoplasm of affected hepatocytes, infiltration of inflammatory cells mainly macrophages and lymphocytes. In conclusion, the oral administration of DEHP at dose 557.41 mg/kg of body weight for 42 days induced degenerative and necrotic irreversible changes in both kidney and liver of Japanese quail that affect their health and production.