Keywords : Heart

Effect of levofloxacin on some body tissues in mice

Rand A. Abdullah; Faten D. Taee; Imad A. Thanoon

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 109-111
DOI: 10.33899/ijvs.2020.126416.1316

Levofloxacin is a third generation of fluoroquinolones family. It is commonly used in the treatment of a several bacterial infections. However, misusing antibiotics impose damage to hepatocytes and myocytes. The present study was conducted to access the effect of levofloxacin on tissue histology in mice taking in consideration dose and duration of exposure. 24 matured male Albino mice were divided into 3 groups, Group G1: was considered as a control group. They received normal saline intraperitoneally / day. Group G2: They received 10.7 mg/kg/day of levofloxacin intraperitoneally for 10 days. Group G3: They received 10.7 mg/kg/day of levofloxacin intraperitoneally for 3 weeks. Microscopic examination of liver sections of group G2 revealed severe congestion of blood vessels in the portal area and central veins with inflammatory cells infiltration. While in group G3, Apoptosis, Degeneration and necrosis of hepatocytes with giant cell transformation were noticed. In addition to kupffer’s cell activation. Heart sections showed moderate congestion of blood vessels with edema in between the myocytes and inflammatory cells infiltration. Group G3 Necrosis with pyknosis of cardiac muscle nuclei was noticed. We concluded that levofloxacin induces toxic effects on liver and heart according to the dose of administration and duration of treatment.

Biochemical and histopathological study of thioredoxin reductase isolation from blood serum in normal and oxidative stress-exposed rats

A.A. Hamdon; L.A. Al-Helaly

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 115-124
DOI: 10.33899/ijvs.2019.163243

The study included investigation of effects of the thioredoxin reductase isolated from serum of human on oxidative stress induced in rats, through histopathological examination of the heart and liver, and the measurement of the biochemical parameters, which included: thioredoxin reductase, creatine kinase, alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin and uric acid. Treating experimental animals with 1% hydrogen peroxide led to a significant increase in thioredoxin reductase, creatine kinase, aspartate aminotransferase and total bilirubin compared to control group, while a significant decrease in: albumin and uric acid, but non-significant in alanine aminotransferase. As well as, a different dose 2 and 4mgl Kg of rat body weight of isolated TrxR with 1% hydrogen peroxide improved parameter levels through decrease oxidative stress that induced in rats. The results of the histopathological examination revealed slight to moderate changes in the heart, while no distinguishing changes were observed in the heart of the group treated with hydrogen peroxide and injected with enzyme in intraperitoneally with 4 mg/ kg of rat weight compared to control group. In the liver, there was observed vascular degeneration and thickening of hepatic capsule as a result of chronic congestion and degeneration in the blood vessels which after treatment with 1% hydrogen peroxide compared to control group, but there was noticeable improvement in the liver of group treated with hydrogen peroxide and injected with the enzyme in intraperitoneally with 4 mg/kg of rat weight, and these results confirm the role of the enzyme in the protection of the body from oxidative stress, the use of the enzyme can reduce the severity of different diseases.

Comparative histopathological effects of aqueous, hexane extracts of Iraqi sweet almond (Prunus amygdalus) with atorvastatin for treating hyperlipidemia induced in mice

L.A. Kafi; N.TH.N. Al- Ezzi

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 1, Pages 13-21
DOI: 10.33899/ijvs.2017.126705

This research was carried out to find out the treatment impact of aqueous and hexane extracts of sweet almond (Prunus amygdalus) on some histopathological indicators of heart, aorta and liver related to hyperlipidemia that induced in mice and compare them with Atorvastatin. Sweet almond was dried and grinded by an electrical grinder to form fine crude powder that extracted by two ways: by using 95% hexane and water by using the distilled water with Soxhlet apparatus. Forty mature mice were randomly divided into 8 groups (5 mice per group) and treated every day for 60 days, the first group was fed and drank normally and regarded as a negative control group, a second group was given polypropylene glycol offered as negative control group, third group was given tap water containing 0.5% of hydrogen peroxide and 1% of cholesterol in the feed for 60 days for induction of hyperlipidemia and offered as positive control group. Hyperlipidemia was induced in the other five groups as in the third group. The treatment of hyperlipidemia was done by using hexane extract at a dose of 500 mg/ kg of body weight and aqueous extract of sweet almond with three different doses (500, 750 and 1000) mg/ kg of body weight and compared with the other group that treated with atorvastatin 20 mg/ kg B.W. as antihyperlipidemic drug. The outcomes discovered that histopathological changes of heart, aorta and liver exposed to hydrogen peroxide and cholesterol revealed congested blood vessels with inflammation relevant cells in their lumen with necrosis of hepatic cells and inflammation relevant cells collected in sinusoids and inner surfaces of the blood vessels and infiltration of macrophages and lymphocytes in the liver moreover to the infiltration of mononuclear cells in the heart while in the aorta showed the vacuolation in the sub intimae and vacuolation and increase the thickness of intimate layer. Furthermore, inflammation relevant cells particularly infiltration around aorta. While therapy with almond extracts and Atorvastatin lead to valuable changes in therapy of damaging happened in the heart, aorta and liver induced with hyperlipidemia. In addition a dose of 1000 mg/kg of aqueous extracts of sweet almond was the best in treatment of hyperlipidemia.