Keywords : Growth hormone

Effect of sodium benzoate on some biochemical, physiological and histopathological aspects in adult male rats

Safa A. Al-Ameen; Eman H. Jirjees; Fadwa Kh. Tawfeeq

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 267-272
DOI: 10.33899/ijvs.2021.129935.1705

Sodium benzoate SB is a white powder, used as preservative and food additive. Biochemical, physiological and histopathological effects of SB been tested in adult male rats. Twenty-four adult albino male rats aged100 day and weighted 250-350 g were used. Animals were divided into 4 groups. The first group considered as control, which received normal saline orally, other groups treated with SB by 300, 400, 500 mg/Kg of body weight respectively for 30 days. At the end of experiment, blood samples were collected from retro orbital sinus. Heart, liver, spleen, kidneys and brain were obtained for weight recording. The results indicated a significant decrease of super oxide dismutase SOD activity and a significant increase of nitric oxide NO level of treated group 500 mg/Kg of body weight. Moreover, findings revealed that there are no significant changes in growth hormone GH activity and body weight. A significant reduction of heart weight of treated group 500 mg/Kg of body weight were observed. The histopathological changes ranged from mild to severe in the brain cortex, as focal gliosis, satellitosis, mild vacuolation and vasogenic edema in treated groups with SB by different doses. Also, some changes were observed in liver represented by congestion of portal vein, mild hydropic degeneration of hepatocytes, stenosis of sinusoids, steatosis and necrosis of hepatocytes in treated groups with SB compared to control group. It concluded that short-term exposure to high doses of SB may be considered an oxidant substance that caused oxidative stress. Furthermore, SB can harm various organs in the body. 

Effect of vitamin C and acetylsalicylic acid supplementation on some hematological value, heat shock protein 70 concentration and growth hormone level in broiler exposed to heat stress

Ashwaq A. Hassan; Rana A. Asim

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 357-363
DOI: 10.33899/ijvs.2019.125950.1195

The goal of the current investigation is to study the effect of vitamin C and acetylsalicylic acid in minimizing the effects of heat stress in terms of hematological values, growth hormone, HSP70 and glutathione. Broiler were randomly distributed into four groups: 1st group was served as the control, the 2nd group subjected to heat stress 40±2 ºC up to 4 hours/day, the 3rd group was subjected to heat stress and vitamin C 360 mg/L via drinking water and the 4th group was exposed to heat stress and acetylsalicylic acid 0.03% via drinking water. The result showed that exposure to heat stress decline in RBCs count, Hb concentration, PCV and percentage of lymphocyte, furthermore elevation in of MCV, heterophils, H/L ratio, and Hsp70 concentration. Administration of vitamin C caused a significant rise RBCs, percentage of lymphocyte and reduces in MCV, MCH value, percentage of heterophils and H/L ratio compared with the heat stress group. Administrations of acetylsalicylic acid were significantly increased the RBCs, and PCV and decrease in MCV, MCH, MCHC values, and H/L ratio compare with the heat stress group. The results did not show a significant change between the tested groups in the levels of glutathione and growth hormone. The study concluded that Vitamin C and acetylsalicylic acid administration as feed additive ameliorating the opposing effect caused by heat stress in the broiler; thus, its administration recommends when there is heat stress exposure.

Effect of supplementation of encapsulated organic acid and essential oil Gallant+® on some physiological parameters of Japanese quails

Hiyam N. Matty; Ashwaq A. Hassan

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 181-188
DOI: 10.33899/ijvs.2019.125732.1142

The current study was conducted to investigate the effects of dietary supplements of organic acid and essential oil Gallant on the growth hormone, glutathione, performance of growth, some biochemical parameters and intestinal histomorphology in quails. Japanese quails one-day-old (n=120) were distributed randomly into four groups included 10 / 3 replicates for each group. The G1, was control group that was fed on a basal diet. Quails of G2, G3, and G4 were fed on a supplemented diet Gallant 300, 600 and 900g/ton,respectively. Administration of Gallant 600 g/ton and 900g/ton to quail led to significant decrease in triglyceride while supplementation with 600 g/ton caused decrease in cholesterol. On the other hand, 300 g/ton caused an increase in final body weight and total weight through the duration of the experiment, as well as a decrease in total feed consumption and the best feed conversion ratio in all supplementation with 300 g/ton caused a significant growth hormone elevation. All feed additives didn't affect the level of glutathione. The addition of Gallant additive groups. Interestingly, the addition of different doses of Gallant to the diet increased villus length and width, crypt depth, villus / crypt ratio, percentage of goblet cell, apparent surface area, and intestinal epithelium thickness compared to the control group. It was concluded that dietary supplementation with different doses of Gallant improved growth hormone, growth performance and intestinal histomorphology in Japanese quails, and dietary supplementation with organic acid and essential oil as alternatives to the growth promoter of antibiotics.