Keywords : Milk

Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk

Zahra K. Saeed; Basil A. Abbas; Rasha M. Othman

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 259-263
DOI: 10.33899/ijvs.2019.125896.1176

The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk. A total of 100 raw milk samples were collected from the different Basrah local markets. All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria. The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%). All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing. The sequencing results showed that 9 strains belong to Lactococcus spp. and 6 strainsbelongto Lactobacillus spp. and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.

The significance of milk ring test for identifying brucella antibodies in cows and buffaloes' raw milk at Erbil governorate, Kurdistan region, Iraq

D.A. Al-Mashhadany

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 395-400
DOI: 10.33899/ijvs.2019.163085

This study was undertaken to monitor Brucella antibodies in the milk of cows and buffaloes in Erbil Governorate, Kurdistan Region, Iraq, using mik ring test (MRT) assay. A total of 210 samples of milk (130 from cows and 80 from buffaloes) were randomly collected from lactating females. The overall prevalence of Brucella antibodies in all the milk samples was 8.6% (18/210). The highest rate was 9.2% found in the cow milk (12/130), while the lowest rate was 7.5% of the buffalo’s milk (6/80). Out of 210 milk samples, only 15 (7.1%) were culture-positive for Brucella; about 7.7% (10/130) and 6.3% (5/80) from cows and buffaloes respectively. In terms of comparison between MRT and standard milk culture method, MRT was found more sensitive (83%), specific (98%), with the accuracy of 97% in comparison to the employed culture approach to detect Brucellosis agents in milk. The results also revealed that 70% and 60% of isolates were Brucella abortus, while 30% and 40% were Brucella melitensis from the milk of cow and buffaloes respectively. The highest rate of frequency for Brucella antibodies according to MRT was found in February (12.1%), while the lowest rate was found in June (5.7%). This study emphasizes that Brucellosis is still a significant public health hazard in the Kurdistan region. The study recommends MRT adoption in routine monitoring of brucellosis in milk collection centre, dairy factories, and farm. Consumers are also recommended to sufficiently heat the milk to destroy this foodborne pathogen before consumption or industrial processing.