Keywords : PRF

Effect of autologous peritoneum and platelet-rich fibrin graft on healing of intestinal anastomosis in dogs

Nebras M. Raffea; Asmaa H. Allawi

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 459-470
DOI: 10.33899/ijvs.2021.130529.1840

In the present study, the effect of autogenous peritoneum graft (PG) with platelet rich fibrin (PRF) was evaluated on intestinal anastomosis healing in dogs. Eighteen local breed adult dogs were anesthetized then jejunal intestinal resection and anastomosis were created on all animals. Animals were divided into 3 equal groups (6 for each). In first group (control): An end-to-end intestinal anastomosis was performed using simple interrupted suture pattern only. In group PG: The anastomosis site was sutured as in control group and was wrapped with PG. Where as in PRF+PG group: The anastomosis site was sutured also as in first group and was wrapped with PRF and PG. Healing process was studied in all above mentioned groups clinically, grossly, histologically and radiographically at 15 and 30 days post operation. Results were revealed that the utilizing of PG caused a valuable influence on anastomosis site healing. This impact was manifested by faster re-epithelization of mucosa, increased collagen deposition, fibroblast proliferation, reduced adhesions, and decreased stenosis degree comparison with control group. Also there was less adhesions, less stenosis degree, increased in epithelization of the mucosa in the group treated with PRF+PG than the group treated with PG alone. In conclusion the current study has shown that autologous peritoneal graft application has a positive and beneficial effect on intestinal anastomosis healing. Also the results highlighted the promising effects of PRF in conjunction with PG on intestinal anastomosis healing. The application of PRF with PG at the intestinal anastomosis site is successful, safe, and highly efficient with no complications.

A comparative study of nano magnesium oxide versus platelets rich fibrin to repair the induced radial fracture in dogs

Yassir F. Abdulmawjood; Moyaser G. Thanoon

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 451-458
DOI: 10.33899/ijvs.2021.130500.1836

This project evaluated the effects of nano magnesium oxide versus platelets rich fibrin on induced radial fracture bone healing. Eighteen males and nonpregnant females of adult local stray dogs, weighing 17.6±0.85 kg and aged 2.08±0.13 years, were used. These trials animals were randomly divided into three groups of equal numbers. In the first group, Control group (C), a transverse radial fracture was induced then immobilized by external fixation as gypsum. In this group, the fracture line was not treated with any bioactive material. In the second group, platelets rich fibrin group (PRF), the fracture line was treated by adding platelets rich fibrin. In the third group, nano magnesium oxide (NMO), the fracture line was treated by adding a suspension of nano magnesium oxide. The radiographic results showed that the fractured bone healing was faster in the second group than in the first and third groups, while the third group was better than the first group. The concentration rates of serum calcium and alkaline phosphatase were increased in the weeks followed the surgical operation. Depending on the radiographic pictures and serial rates of alkaline phosphatase, the second PRF and the third NMO groups were the better-fractured bone healing than the first one. In conclusion, this study revealed that using each platelets rich fibrin and nano magnesium oxide enhanced and improved the healing of the induced radial fracture.