Keywords : RAPD-PCR

Molecular fingerprinting of methicillin resistant Staphylococcus aureus strains isolated from human and poultry in Duhok, Iraq

Hishiyar A Hado; Mahde S Assafi

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126375.1310

Methicillin-resistant Staphylococcus aureus (MRSA) has been recently identified in poultry and farm workers. The aim of this work was to investigate the epidemiological relatedness of MRSA among chickens and farmworker. MRSA isolates (n=50) from human (n=14) and from chikens (n=36) were tested for molecular epidemiological relatedness between human and poultry. RAPD-PCR was carried out for fingerprinting of MRSA isolates genome. Seven genotypes group (A-G) have been identified. All human MRSA were belonging to genotype A. Whereas, chickens MRSA isolates was belonging to different genotype patterns groups (A-G). To conclude, human MRSA was belonging to one genotype pattern but the chickens MRSA strains were belonging to seven genotypes. The genotype pattern A was the most dominant among all MRSA isolates. It is possible that the chickens play an important role for the human exposure to MRSA by direct contact. Further studies are required to address the relatedness between human and chicken MRSA.

Detection of similarity and genetic distance between Iraqi chicken varieties and different standard strains

Thamer A. Ezzulddin; Dhyaa M. Jwher; Sufian A. Dabdoub

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 333-337
DOI: 10.33899/ijvs.2019.126109.1235

Forty-eight wing vein blood samples were collected from different locations of poultry rearing farms and back yard chickens of Nineveh governorate from the of local and exotic chicken. The chicken divided into twelve groups four birds each according to colors and phenotype for the local and exotic chicken respectively. Blood DNA was extracted and amplified by thermocycler apparatus and the electrophoresis was done using 1.2% agarose gel for DNA bands exhibiting. The results showed high genetic similarity within the local chickens ranged between 0.78- 0.96 at an average of 0.88, while it ranged between 0.73- 0.86 at an average of 0.78 in exotic breeds. The degree of similarity between Iraqi and exotic breeds was 0.74-0.88 at average of 0.80. The calculated average of differences among each of Iraqi and exotic chickens and in between were 0.12, 0.22 and 0.20, respectively. However, the genetic distance within the local chicken, exotic breed and in between them was 0.128, 0.24 and 0.21 respectively. The study concluded that the genetic similarity was higher within local chicken groups than those of exotic breeds.