Keywords : Melting curve analysis


The genotype of Entamoeba histolytica in bloody diarrhea samples of humans, cows and sheep

Hassan H. Naser

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 453-458
DOI: 10.33899/ijvs.2020.126135.1242

The present study was carried out to detect the genotype of E. histolytica that found in human fecal specimens and animals feces with Haemorrgic diarrhea by amplifying the SREHP gene, using RT-PCR technique, Cyber ​​green dye and by fusion curve analysis. The study also included molecular detection of amoebic parasite species using Nested-PCR technology. The study recorded presence of parasites E. histolytica; E. dispar; E. bovis with total infection rates 82.9, 26.8, 4.9%, respectively. The study revealed the presence of E. histolytica parasite in five different genotypes (I, II, III, IV, V) with rate presence 9.75, 53.65, 19.5, 9.75, 7.3%, respectively. In conclusion, there are five genotype of E. histolytica, in human and animals, most of these genotypes may be infect any host, E. bovis was recorded in sheep and cows.