Keywords : Bionanocomposite

Characterization and In Vitro evaluation of a novel coated nanocomposite porous 3D scaffold for bone repair

Sahar Ibrahim; Saffanah Mahmood; Intan Shameha Binti Abdul Razak; Loqman Mohamed Yusof; Zaid Khudhur Mahmood; Fufa I. Gimba; Md Zuki Abu Bakar Zakaria

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 157-173
DOI: 10.33899/ijvs.2019.125548.1068

The aim of this study is to tissue engineer a 3D scaffold that can be used for load bearing segmental bone defects (SBDs) repair. Three different scaffolds were fabricated using cockle shell-derived CaCO3 aragonite nanoparticles (CCAN), gelatin, dextran and dextrin with coated framework via Freeze-Drying Method (FDM) labeled as 5211, 5211GTA+Alginate, 5211PLA. Scaffolds were assessed using Scanning Electron Microscopy (SEM). The cytocompatibility of the organized scaffolds was assessed using cells multiplication and alkaline phosphatase (ALP) concentration via In Vitro cell culture using human Fetal OsteoBlast cells line (hFOB). The results showed a substantial difference in ALP concentrations between the cultures of different scaffolds leachable medium during the study period. The biological evaluation also showed that three scaffolds did enhanced the osteoblast proliferation rate and improved the osteoblast function as demonstrated by the significant increase in ALP concentration. Engineering analyses showed that scaffolds possessed 3D interconnected homogenous porous structure with a porosity ranging 6%-49%, pore sizes ranging 8-345 µm, mechanical strength ranging 20-65 MPa, young’s modulus ranging 166-296 MPa and enzymatic degradation rate between 16%-38% within 2-10 weeks. The in vitro evaluation revealed that the scaffold 5211, 5211GTA+Alginate and 5211PLA fulfill all the main requirements to be considered as an ideal bone replacement.

In Vivo evaluation of the novel nanocomposite porous 3D scaffold in a rabbit model: hematology and biochemistry analysis

S. J. Mahmood; Md. Z. Zakaria; I. Sh. Abdul Razak; L. M. Yusof; A. A. Abubakar; Z. K. Mahmood; M. Q. Bin Ab Latip

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 2, Pages 219-230
DOI: 10.33899/ijvs.2019.153853

Issues of safety are very crucial with biomaterials and medical devices. Sixteen male New Zealand White rabbits equally into four groups: Group A, rabbits had part of their radial bone (2 cm, mid shaft) and left empty as a control. Group B, rabbits were implanted with scaffold 5211. Group C, rabbits were implanted with scaffold 5211GTA+Alginate. Group D, rabbits were implanted with 5211PLA. All scaffolds were prepared by freeze-drying method. Blood samples were collected at day 0 and 1st, 2nd, 3rd, 4th and 8th week after implantation. The blood examination included complete hemogram and certain serum biochemical parameters. The results showed that there was no significant difference (P>0.05) among each treatment group in comparison with control group (day 0). However, red blood cells, hemoglobin, packed cell volume, mean corpuscular hemoglobin concentration, monocyte, plasma protein, inorganic phosphate, sodium, chloride and urea were significantly increased (P<0.05) among treatment groups at week 8. An abnormal architecture of viscera was observed in all animals, thus indicating a form of toxicity related to the degrading scaffold materials. The severity of histopathological lesions in viscera was not coated polymers dependent nor development materials. In conclusion, implantation of 5211 scaffold with or without coated framework has a significant impact on histopathological and certain hematological and biochemical parameters.