Keywords : Reproduction

Novel single nucleotide polymorphism in the prolactin gene of Awassi ewes and its role in the reproductive traits

Tahreer M. Al-Thuwaini

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 3, Pages 429-435
DOI: 10.33899/ijvs.2020.126973.1423

This study was conducted to determine the genetic variation of the prolactin (PRL) gene in 5' flanking region and its role with several reproductive traits in Awassi sheep. One-hundred six Iraqi sexually mature and healthy Awassi ewes that aged between 2 and 2.5 years were included in this study. Ewes were classified into two the main divisions: ewes producing twins and ewes producing single offspring. Two genotypes (AA and AT) were observed in Awassi sheep. While genotype AA was detected in Awassi ewes that produced twins, genotype AT was detected in ewes that produced a single offspring. The sequencing reactions identified nine SNPs in the prolactin gene at the 5′ flanking region in Awassi sheep, differing from the prolactin reference sequence (GenBank accession number X16641.1). Genotype AT possessed one single nucleotide polymorphism SNP substitute comparison with the AA genotype in Awassi ewes. The association analysis revealed that the AA genotype is characterized by significantly higher levels of the progesterone concentration, twinning ratio, fecundity, and prolificacy than the AT genotype. In conclusion, a new SNP (g.1209 A>T) was discovered within the ovine flanking region which potentially influences prolactin gene expression. These results showed that the genotype AA associated with high prolificacy of Awassi sheep may be used as a selection criterion for improving the reproductive performance of Iraqi Awassi sheep.

Ovarian morphometric evolution in two consecutive estrous cycles of female rats treated with steroid-free bovine follicular fluid antiserum

Jabber A. Al-Sa’aidi; Areej H. Al-Charack

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 265-271
DOI: 10.33899/ijvs.2019.125923.1186

The current study aims to investigate the role of steroid-free follicular fluid antiserum on ovaries morphometric growth and development in virgin female rats at two sequential estrous cycles. Bovine follicular fluid aspired from graafian follicles, steroids were removed by treatment with activated charcoal, in order to obtained steroid-free bovine follicular fluid (S-FBFF) which was used for immunization of male rabbits to prepare S-FBFF antiserum (S-FBFF-ab). Sixty virgin female rats were assigned into control and treatment groups (30 each), intraperitoneal injected with a single dose of distilled water 100µl/rat and S-FBFF-ab 100µl/rat at late metestrus, respectively. At the estrus phase of the first and second estrous cycle, 15 females from each group of each cycle were anesthetized and ovarian samples were obtained for histological examination. In comparison with control, the results of S-FBFF-ab treated female rats revealed a significant increase of relative ovaries and uteri weights at both estrous cycles. Morphometric examination showed progressive ovarian proliferation at the first estrus phase in S-FBFF-ab treated female rats through elevation of the number of primaries, graafian, and total follicles. In conclusion, passive immunization against endogenous inhibin using S-FBFF-ab could augment the reproductive fecundity through increase ovarian growth and development.

Effect of β-mannanase, Lysolecithin and probiotic on some reproductive performance and hormone profile in female quail

Hadel M. Hameed; Fadwa Kh. Aga; Saeb Y. Abdulrahman

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 87-93
DOI: 10.33899/ijvs.2019.125587.1097

The aim of this study to evaluate the effect of β-mannanase. Lysolecithin and Probiotic on some reproductive performance and hormone profile in female quail. Six hundred one day - old quail birds were randomly divided to four treatments (60 birds/treatment) with 3 replicates for each group treatment (20 birds / replicate) and at three duration periods. The first period 1-7 weeks, second period 7 - 13 weeks, third period 1-13 weeks. The group of study were control was fed on a standard ration. the second, third and fourth treatments were given β-mannanase. Lysolecithin. probiotic 0.5 g /kg ration respectively. Blood samples wear taken for blood serum analysis. including estimation of follicle- stimulating hormone and luteinizing hormone. The results revealed that the treated groups showed a significant increase in relative weight of the ovary and oviduct compared with control group. β-mannanase and probiotic groups showed a significant increase in the oviduct length compared with the control and Lysolecithin groups. There was a significant increase in the numbers of growing and mature follicles and weight of large follicle in all treated groups compared with control group. The 3rd period showed a significant increase in the relative weight of ovary. oviduct and numbers of growing follicles. while the 2nd and 3rd period showed a significant increase in the oviduct length. About the interaction between treatments and periods. the best result appeared in the ovarian relative weight in the 3rd period for probiotic and β-mannanase. the 3rd period of probiotic showed a significant increase in the oviduct relative weight and for the oviduct length at 2ed and 3rd period for probiotic and β-mannanase. while 3rd period of probiotic show best interaction in the numbers of growing follicles, as 2nd and 3rd periods showed better increase in the mature follicle's numbers and for large follicle weight in 1st and 2nd period of probiotic. The result also showed a significant increase in the FSH and LH level in all treated groups compared with control group. The 2nd and 3rd period were better significantly in the level of FSH and LH. Probiotic at 2nd and 3rd period showed a significant interaction on the level of FSH. On the other hand, 2nd and 3rd period for the β-mannanase and probiotic showed a better significant in the LH level. In conclusion. β-mannanase. Lysolecithin and probiotic supplementation to quail ration improved the hormonal status and enhance reproduction.

Reproductive hormones profile of Iraqi Awassi ewes immunized against synthetic inhibin-α subunit or steroid-free bovine follicular fluid

S.S.A. Kafaji; J.A.A. Al-Sa; aidi; K.K. Khudair

Iraqi Journal of Veterinary Sciences, 2017, Volume 31, Issue 2, Pages 123-128
DOI: 10.33899/ijvs.2017.145609

The current study was conducted to investigate the impacts of active and passive immunization against synthetic inhibin-α subunit and steroid-free bovine follicular fluid, respectively, on reproductive hormones profile out of breeding season in Iraqi Awassi ewes. Follicular fluid was aspired from mature bovine follicles, treated with activated charcoal, used for immunization of male rabbits, and obtaining of SFBFF antiserum. Forty non-pregnant Awassi ewes were allocated into 4 groups (n = 10 each). At day 38 of experiment, ewes were treated with intra-vaginal sponge impregated with medroxyprogesterone acetate 60 mg for 12 days. Ewes were treated at 0, 28 and 50 days with 4, 2 and 2 ml of normal saline (control; C-ve), 400, 200 and 200 µg of ovalbumine (C+ve), 400, 200 and 200 µg of inhibin (SI group), and 4 ml of normal saline at 0 day, and 4ml and 2ml of SFBFF antiserum, at 28 and 50 days, respectively (AI group). Blood samples were collected at 24 and 48 hours before and after sponge withdrawal for assessment of FSH, LH, inhibin-B, Activin-A, E2 and P4. Before sponge withdrawal, FSH level increased in SI ewes, whereas only after sponge withdrawal, FSH, LH, activin-A and E2 levels increased in SI and AI ewes. Opposite results were shown of inhibin-B level. In conclusion, active or passive immunization against inhibin in Awassi ewes could augment reproductive functions out of breeding season in Iraqi Awassi ewes.