Keywords : Histopathology
Does Baclofen induce changes in testicular histology and seminal fluid analysis in rat?
Iraqi Journal of Veterinary Sciences,
In Press
DOI:
10.33899/ijvs.2020.126894.1411
Baclofen has a central acting effect, so clinicians have been utilized it chiefly for treating the spasticity of spinal origin. Nowadays, off-labeling use of baclofen with high doses is frequently increased. Despite of the extensive research studies on the effectiveness of baclofen, the reports on its histological effects on testes and on sperm parameters we insufficient. This work aims to assess the histological influences of baclofen on rats' testes and on several sperm characteristics after administration for 8 weeks. Twenty-two male rats at age of peripuberty (8weks) that were categorized into two groups. Group I (control group) includes 10 rats which were gavaged with 1 ml/day of distilled water daily. Group II (baclofen's group) includes 12 rats which were received baclofen 14.5 mg/kg for 8 weeks via gavage. At the end of the designed work, euthenization was done and the testes were excised from each rat, the epididymis samples were obtained and prepared for examination under light microscope. This study revealed that rats that were administered with 14.5mg/kg/day of baclofen for 8 weeks showed changes in the sperms parameters with several testicular histological alterations in their sections in comparison with those of controls. The percentage of the live sperms of rats (baclofen's group was lower than that of control group). Features of sloughing inside seminiferous tubules' lumen are frequently seen among these sections with mild decrease in the diameter of seminiferous tubules. Evidence of decreased Johnsen's scores (spermatogenic index -SI) was noticed in some sections, and decreasing of the frequency of seminiferous tubules that possess spermatozoa was obviously noticed. Moreover, disorganization of seminiferous tubules is shown in these sections. In addition, sections of rats of baclofen group revealed features of necrosis of seminiferous tubules, presence of degenerated spermatogenic layer, features of apoptosis, and presence of gaps in the seminiferous epithelium of degenerated tubules. Different alterations of sertoli cells as vacuolation, and detachment sertoli cells are noticed in sections of rats that were administered with baclofen. The chronic administration of rats with 14.5 mg/kg with baclofen induced effects on sperm parameters and testicular histology. however, the testicular histological alterations were more obvious. Further works are needed to clarify mechanism that stands behind these effects.
Histopathological effects of experimental exposure to lead on nervous system in albino female rats
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 45-48
DOI:
10.33899/ijvs.2019.126248.1273
Lead toxicity is a common health issue. Lead (Pb) is harmful to vital organs of body particularly the nervous system. This study aimed to estimate the effects of lead on the cerebellum, cerebrum and spinal cord in rat model, focusing on histopathological changes. 24 female mature albino rats of 200-300g randomly divided into 2 groups, the first is the control, and the second group were treated with lead acetate at dose 30mg/kg B.W. for 30 days. Microscopic examination revealed degeneration and necrosis of Purkinje cells and molecular cells and decrease in the number of granular cells and molecular cells also observed. Some Purkinje cells lost axons and shrunken and some areas showed depletion of Purkinje cells. Congestion of blood vessels with perivascular cuffing of mononuclear inflammatory cells, hemorrhage, neurophagia, glial nodules were observed in the brain parenchyma. Demyelination reported in white matter, with microglial proliferation around vertebral canal of spinal cord. This study referred to the increased risk of central nervous system damage due to the exposure to lead.
Pathological study of neoplasms surgically excised from animals attended the veterinary teaching hospital
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 9-14
DOI:
10.33899/ijvs.2019.126188.1260
This study aimed to investigate the occurrence and histopathological features of neoplastic conditions in different species of animals that attending the Veterinary Teaching Hospital, College of Veterinary Medicine, University of Mosul. Samples had been collected from animals with variable neoplastic lesions. The results of this study showed that the total number of the excised tumours was 36, those were noticed in cattle 30.6%, sheep 22.2%, dog 10.4%, poultry 13.9%, cat 11.1%, and goat 2.8%. The tumours were diagnosed as squamous cell carcinoma 22.22%, fibroma 19.44%, adenoma 13.89%, pulmonary adenomatosis and fibrosarcoma 11.11%, leucosis and lymphoma 8.33%, thymoma, and transmissible venereal tumour 2.78%. We concluded from the current study occurrence a different type of tumours which was malignant or benign in different species of animals.
A histological study on the effect of imatinib on the rats' testis after early postnatal exposure
Iraqi Journal of Veterinary Sciences,
2021, Volume 35, Issue 1, Pages 85-92
DOI:
10.33899/ijvs.2020.126342.1303
The safety region of imatinib, and markedly its role in testicular toxicity has been studied in a controversial manner in current years. This study was designed to address the repercussion of administration of groups of pups with imatinib mesylate (at neonatal or infantile periods) using histological analysis of their testes in several end points and in adulthood. Intact pups (albino-male) exposed to 200 mg/kg of oral imatinib once daily at neonatal and early infantile period on PND 1 to PND10 (for ten days). All experiments performed with age and weight matched control which administered with Distilled water. Pups were into categorized into 4 subgroups, according to the ages of euthanasia: 15 days postpartum (PND); 40days postpartum; 70 days postpartum and 140 days postpartum. The histological analysis was conducted in blind fashion after staining with (Harris Hematoxylin and Eosin stain. Ten randomly selected testicular sections from each rat were analyzed qualitatively and quantitavely. In addition, Johnsen' scores were used to analyze the effect of drug on spermatogenesis. Data were recorded and value is considered as significant when it P
Improving light microscopic detection of collagen by trichrome stain modification
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 2, Pages 273-281
DOI:
10.33899/ijvs.2019.126176.1256
In present study we aimed to introduce modifications in classical protocol applied to stain collagen fibers by Masson's trichrome stain, in order to decrease time and concentration of different steps and chemical in this traditional protocol. The experiment design contains a series of successive amendment steps based on previously successful steps, in which every step where be modified to get the best result, then the next step of staining protocol will be modified in the classical Masson's trichrome staining protocol in a samples of tissue from liver, heart and intestines of sheep diagnosed with chronic fasciolosis. The result showed that the new modified protocol gives the same staining efficiency to collagen fiber when we used Harris hematoxylin as a nuclear strainer or even excluded this stain from staining protocol. In conclusion this new modified staining protocol give a perfect staining reaction to collagen fibers in pathological samples which is similar to that obtained by Masson's trichrome stain, also this new modified procedure is less time consuming and less toxic effect on human and environment than other trichrome stains, and can be easily conducted either by the technician or the pathologists.
Histopathological effect of fluoxetine drug on the brain of pregnant mice and their embryos
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 71-76
DOI:
10.33899/ijvs.2019.125467.1006

Diagnosis and histopathological study of avian influenza virus-H5 (AIV-H5) in broiler farms
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 101-107
DOI:
10.33899/ijvs.2019.125646.1120

Toxicological and neurobehavioral effects of chlorpyrifos and deltamethrin insecticides in mice
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 189-196
DOI:
10.33899/ijvs.2019.125738.1144

Incidence of cutaneous and subcutaneous tumors of dogs from Baghdad city: Clinical, cytological and histopathological features
Iraqi Journal of Veterinary Sciences,
2020, Volume 34, Issue 1, Pages 129-137
DOI:
10.33899/ijvs.2019.125624.1111

Study of histopathological and biochemical effect of Punica granatum L. extract on streptozotocin -induced diabetes in rabbits
Iraqi Journal of Veterinary Sciences,
2019, Volume 33, Issue 2, Pages 189-194
DOI:
10.33899/ijvs.2019.125523.1045
This study was undertaken to determine the antidiabetic effects of oral administration of Punica granatum L. extract on serum and tissues of streptozotocin induced diabetic rabbits at 100 mg/kg. The present study was carried out at the Faculty of veterinary Medicine, Tikrit University, from February to August 2017 for 10 weeks. For this purpose, 30 rabbits were randomly separated into three groups, each containing 10 animals: Group 1, healthy control rabbits; Group 2, diabetic rabbits received streptozotocin (STZ, 65 mg/kg); Group 3, diabetic rabbits treated with PS extract (the 100 mg PS+1 ml DW) for 21 days. At the end of experiment, blood samples were taken for measuring serum biochemical parameters. For histopathological evaluation, sections of kidneys were fixed in 10% buffered formalin and 5micron thick sections with H&E stain were prepared using routine histopathological techniques. The treatment revealed that PSE extract significant decreased serum glucose throm-bospondin-1, nitric oxide, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase alkaline phosphatase, and C-reactive protein in diabetic treated rabbits as compared to diabetic rabbits. Histopathology of kidney showed lesions similar to human glomeruloscleroses, glomerular membrane thickening, arteriolar hyalinization and tubular necrosis. From the above one can conclude that PSE extract possess nephroprotective effect in experimentally induced diabetic rabbits.
Physiological and histological effects of broccoli on lead acetate Induced hepatotoxicity in young Male albino rats
Iraqi Journal of Veterinary Sciences,
2019, Volume 33, Issue 1, Pages 21-26
DOI:
10.33899/ijvs.2019.125528.1050
The aim of this study was to investigate the ameliorative effect of Broccoli against lead acetate (PbA) hepatotoxicity by some physiological and histological indicators. The results showed that intraperitoneal (I.P.) injection by 12 mg/kg body weight of lead acetate once a week for 8 weeks led to an increase in the activity of Glutamate pyruvate transaminase (GPT), Glutamate oxaloacetate transaminase (GOT), Alkaline phosphatase (ALP), Acid phosphatase (ACP), Total serum protein (TP) and Total serum bilirubin (TSB). Liver's histological sections of lead acetate injected rats showed infiltration of inflammatory cells with sinusoid dilation, necrosis, and apoptosis of Kupffer cells. Broccoli has an ameliorative effect, that the physiological parameters and histological examination have been showed an improvement. In conclusion, lead acetate produces hepatic disorder and the potential use of Broccoli as a source of natural antioxidants or nutraceuticals protects against lead acetate hepatic toxicity.