Keywords : lactic acid bacteria

Physiological effects of lactic acid bacteria against melamine induced toxicity in female albino rats

Ali H. Jameel; Mohammed J. Mohammed; Manal S. Mahdi; Karkaz M. Thalj

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 1-7
DOI: 10.33899/ijvs.2020.126183.1259

The aim of this study was to investigate the ameliorative effect of two type of lactic acid bacteria Lactobacillus casei and Lactobacillus acidophilus against melamine toxicity by some physiological indicators in mature female rats after 21 days. In this study using 35 of female mature rats and divided randomly into seven groups each group contain five animals. The results showed that melamine caused a significant decrease in the organs weights liver and spleen and increase in kidney weight with increase of melamine concentration. Also showed to decrease in value of hemoglobin, red blood cells, white blood cells, lymphocyte and platelets, while the values of granules were increasing with increase of melamine concentration as compared with control group. Also found that the addition of melamine led to increase in cholesterol, low density lipoproteins and blood glucose, while the values of triglyceride and high density lipoproteins was decreased with increase of melamine concentration. The addition of two types of lactic acid bacteria L. casei and L. acidophilus led to decreasing the negative effect of melamine on the values of all the parameters determined.

Molecular identification and phylogenetic analysis of lactic acid bacteria isolated from goat raw milk

Zahra K. Saeed; Basil A. Abbas; Rasha M. Othman

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 259-263
DOI: 10.33899/ijvs.2019.125896.1176

The aim of this study was to identify the genetic diversity of lactic acid bacteria (LAB) isolated from the local goat's milk. A total of 100 raw milk samples were collected from the different Basrah local markets. All the samples were cultured in the De man, Rogosa, and Sharpe (MRS) medium which enhances the growth of lactic acid bacteria. The result of the study showed that the only 64 lactic acid bacteria isolated gave the Gram-positive and catalase-negative were 64 (64%). All the suspected isolates were detected and identified by using polymerase chain reaction (PCR) targeting the 16S rRNA gene and DNA sequencing. The sequencing results showed that 9 strains belong to Lactococcus spp. and 6 strainsbelongto Lactobacillus spp. and all tested isolates had similarity over 99% with those recorded in the GenBank of The National Centre for Biotechnology.