Keywords : Newcastle Disease

Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test

Fanar A. Isihak; Salah M. Hassan; Balqees Z. Shaker; Yasir A. Salih

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 295-299
DOI: 10.33899/ijvs.2019.125931.1189

The study period carried out from 25 April 2018 till 21 May 2019 through the rearing and production period including totally of 24000 birds (20800 females, 3200 males). The number of tested blood samples was 452 divided to 255 samples at the rearing period, 143 samples at the production period and 54 samples of offspring. The results of antibodies titer in the sera of non-vaccinated broiler breeders obtained by ELISA showed the maternal derived antibodies titer for 28 samples at 0-5 week/day of age was 5716±612.7, this titer decreased gradually at 3-1 week/day age till to 1075±234) Then the titer was elevated increasingly after vaccination with both live attenuated and inactivated vaccines and reach to peak 37512±2049.4 at 20-1 week/day age. Whereas the bimodal graduation of antibodies titer showed at production period till to end of study. The mean of maternally antibodies titer in the tested sera of the offspring chicks 0-1 week/day that hatched from parent flocks at 32, 39 and 48 weeks of age was 9012±872.4, 6591±368.1 and 4831±982.7 respectively. Thus, we concluded the repetitive vaccination of broiler breeders flock with live vaccine as well as inactivated vaccine is very necessary in endemic areas and ELISA is a good serological test for following, checking and monitoring of immune status of poultry flocks periodically.

Effect of hyperimmunized egg yolk on maternal immunity of Newcastle disease vaccine in broiler chicks

A.Z. Al-Zubeedy; M.Y. Al-Attar

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 35-38
DOI: 10.33899/ijvs.2020.167133

Hyperimmunized egg yolk (HIY) was produced in two layer hens by four successive immunization with live attenuated Newcastle disease vaccine (NDV) by seven days intervals for each vaccination process. Fifty broiler chicks were used for treatment with hyperimmunized yolk. They were divided into five groups. The first group was treated at 14th days of age by orally and intramuscular injection (five chicken for each route). The second group was boostered with (HIY) after 7 days of first dose at14 days of age. The third group as that of the first group but treated at 21st day of age. The fourth group was treated at 21st days and boostered after 7 days. The fifth group was ten chicks remain without any treatment used as control comparison of all groups. Immune response was measured using HI technique.The results showed that the group of 14th day of age with booster dose gave high antibody titer by intramuscular injection (second group).

Effect of Synertox® on broiler health and performance during aflatoxicosis

A.M. Shareef; E.K.S. ar Om

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 27-34
DOI: 10.33899/ijvs.2020.167132

Eighty, one-day-old commercial male broiler chicks (Ross 308), were distributed to four dietary treatments with two replicates of 10 chicks each, to study the effect of Synertox® on broiler performance during aflatoxicosis. Birds were reared for 42 days. All birds were fed on diet with or without aflatoxin (AF) in feed, and with or without Synertox (ST) (enzymes, organic acids and yeast extract) in water. The treatments were as follow: T1 (0 ppm AF and no ST); T2 (2.5 ppm AF and no ST); T3 (0 ppm AF and 0.5 ml/l ST); T4 (2.5 ppm AF and 0.5 ml/l ST). Body weights and feed intake were recorded weekly. At 42 of bird’s age, five birds were randomly selected for estimation of antibody titres against Newcastle disease (ND; for calculation of the relative weight of bursa of Fabricius, thymus and spleen as well as for scoring liver and kidney lesions. The results show that the total body weight gain through 42 days of rearing period that the gain was highest in T3. In the second order was the weight of the control group (T1), while in the third order was T4. The worst body weight gain was reported in T2, in which chicks fed AF. The same picture in body weight gain was obtained with feed consumption and feed conversion ratio. AF had a significant negative effect on the liver parenchyma of broiler chicks in treatment 2, by changing liver colour from mahogany to enlarged muddy or even to yellowish discolouration, with friable consistency with sub capsular haemorrhages. The addition of Synertox® was effective in restoring the normal red brown liver colour. Kidney were also affected by feeding AF (T2). They were enlarged, swollen and pale in colour. All lymphoid organs tested, thymus, bursa of Fabricius and spleen were negatively affected by feeding AF in broilers at 42 days of age. Synertox® was effective in counteracting the negative effect of AF on relative bursal weight, thymus and spleen relative weights. Aflatoxin had detrimental negative effect on the ND ELISA mean antibody titre. A significant restoring of ND antibody titre to those of control group (T1) was recorded by addition of Synertox® to the drinking water of broilers in (T4) compared with the AF fed T2 group. From the studied parameters it could be concluded that Synertox® could be used in counteracting the negative effects of AF on health and performance of broiler chicks.