Keywords : FMD

Evaluation of cardiac enzymes and acute phase response as biomarkers for rapid diagnosis of myocarditis in calves with FMD

Kamal M. AlSaad; Hasanin N. Al-Autaish; Jihad A. Ahmed

Iraqi Journal of Veterinary Sciences, Volume 34, Issue 1, Pages 31-37
DOI: 10.33899/ijvs.2020.163584

Troponin-I, homocysteine, Creatine kinase-myocardial band, lactate dehydrogenase and acute phase response had been evaluated in calves with myocarditis due to FMD. The study was conducted on 52 local breed calves 1-6 months old and from both sexes, their dams have no history for vaccination against FMD and show classical foot and mouth disease signs. Ten clinically healthy calves of the same ages were considered as controls. Suspected calves neither show oral blisters, rope salivation, nor foot lesions. Diseased calves showed signs of dullness, in activity, panting with mouth breathing, unable to suck, recumbency, However, five of diseased calves were died within 24-72 hours and on macroscopic examinations of autopsied animals, necrotic myocarditis with pale foci with a zone of hyperemia which were present on the papillary and ventricular cardiac muscles, moreover, on histopathological examinations there were severe inflammatory cells infiltration in the interstitial of myocardial fibers with obvious area of coagulation of myocardial fibers and marked area of hyalinization, furthermore, severe mononuclear cells infiltration, mainly lymphocytes, with few neutrophils closed to necrotic myocardial fibers were also detected. Diagnosis of FMD virus was confirmed by using commercially NSP ELISA kits for foot and mouth. A significant increase (p>0.05)was encounteredin body temperature, respiratory and heart rates in diseased animals than in controls, Furthermore, abnormal cardiac sounds (organic murmurs) were indicated on auscultation of the heart. Results of hematological parameters shown a significant increase indicated in ESR values of diseased calves than in controls, moreover, total leukocyte count was increased significantly with significant lymphocytosis. Furthermore, the results were also showed significant increase in values of serum cardiac troponin, homocysteine, creatine kinase-myocardial band, lactate dehydrogenase, haptoglobin and fibrinogen in seropositive calves for FMD compared with controls. It can be concluded that determination of cardiac biomarkers and acute phase response concentration in calves with myocarditis can considered as a guide to quantify early heart damage.

Reverse transcription-polymerase chain reaction (RT-PCR) based detection and economic impact of foot-and-mouth disease in District Faisalabad, Pakistan during the year 2015

W. Ali; M. Habib; R.S.A. Khan; M.A. Zia; I.U. Khan; U. Saliha; M. Farooq; M.S. Shah; H.M. Muzammil

Iraqi Journal of Veterinary Sciences, Volume 31, Issue 1, Pages 1-6
DOI: 10.33899/ijvs.2017.126703

The aim of this study was to evaluate the economic impact of the disease by using milk production records and to determine the serotypes circulating in the region during 2015. Sampling was done from different outbreaks initially on the basis of clinical signs and later reverse transcriptase-polymerase chain reaction (RT-PCR) was employed for the conformation of FMDV genome. Out of total 88 samples, 73 were found positive which were then serotyped into type O (n=44), Asia1 (n=18) and A (n=06). The economic impact was analyzed by recording milk loss at four affected farms. Their average milk yield was observed 9.2 liters before the onset of disease that decreased dramatically after the disease. Milk loss of 225 and 195 liters was recorded for buffalo and cattle respectively, during 70 days of the study period.