Keywords : Hydatid cyst

Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq

R.N. Hamoo; nashaat Ghalib Mustafa; S.A. Abdulraheem

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 341-345
DOI: 10.33899/ijvs.2019.162965

Echinococcus granulosus (E. granulosus) is a dog tapeworm cestoda; it is larval stage responsible to cystic echinococcosis, one of the most common and dangerous worldwide zoonotic parasitic disease. The aim of this study was the molecular identification of the local strain of E. granulosus isolated from sheep liver slaughtered in the principal abattoir of Aqrah city, Northern of Iraq during Jun-Nov. 2017. In this study, 37 sheep liver infected by E. granulosus, 12 of high DNA purity fertile (have protoscolices) cyst of them were considered. A molecular study conducted on the mitochondrial NADH dehydrogenase 1 (nad1) gene. Results demonstrated that E. granulosus isolates were sheep strain (G1) genotype, with fascinating highly corresponding 95% and 96% to global isolates, particularly to north African and Mediterranean countries, by employing phylogenetic tree analysis. So, the isolates of our project were deposited in Genbank (accession No. MG792129). This study findings provide that the local isolates of E. granulosus from sheep liver in Aqrah city, Northern of Iraq are loyally equivalent to global strains and isolates, in addition, nad1 gene considers a perfect biomarker in a molecular identification and phylogenetic study of this parasite.

Incidence of hydatidosis in slaughtered livestock at Mosul, Iraq

M.T. Jarjees; H.S. Al-Bakri

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue 1, Pages 21-25
DOI: 10.33899/ijvs.2012.46893

A study on the occurrence and seasonal incidence of hydatid cysts of sheep, goats and cattle was carried out during 2008 and 2009 by weekly regular visits to Mosul abattoir and other areas i.e. outside abattoir. All slaughtered livestock were of local breed, of both sexes, originated from various areas of Mosul and were of different ages having non descriptive features. Visual inspection and palpation of the lesions were followed in this study. Of 4800 sheep, 960 goats and 720 cattle were examined, 96 sheep, five goats and four cattle were found to harbour the cysts representing infection rate of 2%, 0.52% and 0.55%, respectively. The lowest seasonal incidence was observed in winter for sheep (3.16%) and goats (1.25%). The lowest level of incidence was 0.16% for sheep and 0% for goats was noticed in summer. However, in cattle no infection was taken place in winter and autumn but 1.11% infection rate was equally seen in summer and spring. The preponderant site of cyst was the liver in sheep (46.8%) and goats (40%). In cattle the commonest location of the cyst was the lung (50%) followed by mixed site of liver and lung (25%) and liver (25%). The results indicated that only fertile cysts were present in the sheep representing 83.33% of fertility percentage. The number of cysts in the infected organs ranged between 1-16, 1-6 and 1- 10 for sheep, goats and cattle, respectively. It can be concluded that only sheep play a major role in dissemination of hydatidosis. However, being anthrozoonotic, potential risk may be increased due to incorrect disposal of infected offal with unhygienic slaughter protocols.