Keywords : Encephalitozoon cuniculi


Pathological study of intrauterine infection to embryos by Encephalitozoon cuniculi spores in pregnant mice

Saevan Saad Al-Mahmood; H.I. Al-Sadi

Iraqi Journal of Veterinary Sciences, Volume 30, Issue 2, Pages 49-54
DOI: 10.33899/ijvs.2016.121383

This study aimed to investigate pathology of oral E. cuniculi infection during pregnancy in pregnant mice and embryos. A total of 40 pregnant mice at first day of gestation were divided into two groups, first group were infected orally by E. cuniculi sores of 107 spores/ mice, second group left without any treatment. At 18th days of gestation all pregnant mice were euthanized. Gross pathology finding in pregnant mice of infected group included congestion of liver and lung, the embryos lesions consisted from enlargement of head and abdomen. Histological lesions in pregnant mice of infected group consisted of hepatic non-suppurative granulomatous lesions with E. cuniculi spores aggregation with lymphocytic infiltration, the lungs lesions consisted of infiltration of lymphocytes with E. cuniculi spores, kidney lesions composed from degenerative and necrotic changes in renal tubules, brain lesions consisted from lymphocytic infiltration with increase in number of glial cells, while intestine tissue sections showed hyperplasia of lymphatic tissue with present of parasitic vacuoles at tips of villi, the placenta exhibited E. cuniculi spores with hyperplasia of trophoblast in chorionic villi, while histological lesions in embryos showed lymphocytic infiltration around alveoli with hyperplasia of lymphatic tissue around bronchioles with absent the normal architecture of hepatic cords and vacuolation of hepatocytes with hyperplasia of lymphocytes in white pulp of spleen. This study provides insight into the pathology of E. cuniculi infection in pregnant mice and their embryos, also supports the hypothesis of intrauterine transmission of E. cuniculi infection to embryos during pregnancy period.

The first unequivocal report of Encephalitozoon cuniculi in rabbits and Encephalitozoon intestinalis in cattle in Mosul city-Iraq

Saevan Saad Al-Mahmood; Hafidh I. Al-Sadi

Iraqi Journal of Veterinary Sciences, Volume 29, Issue 1, Pages 0-0
DOI: 10.33899/ijvs.2015.116859

The aim of present study was to report spontaneous Encephalitozoon cuniculi (E. cuniculi) infection in rabbits and Encephalitozoon intestinalis (E. intestinalis) infection in cattle in Mosul city - Iraq. Fecal samples were collected randomly from 142 rabbits and 115 cattle during the period from June, 2012 to June, 2013. Thin smears were prepared from the fecal samples and stained with different fecal stains, as well as the indirect immunofluorescent antibody technique. Infected rabbits were necropsied and pathology of the infection was described. The result showed that microsporidiosis was detected in 41 (26.9%) rabbits and in 12 (10.4%) cattle. E. cuniculi spores were identified in 31 out of the 41 rabbits (73.2%) and E. intestinalis in 7 out of the 12 cattle (58.3%). Infected rabbits showed vasculitis, granulomatous encephalitis, chronic interstitial nephritis, granulomatous hepatitis, cholangitis, vasculitis and non – suppurative pneumonia, infiltration of mononuclear cells in most layers of the intestines, and hyperplasia of lymphoid tissues. E. cuniculi spores were seen in the liver, kidneys and lungs. This study considered the first one that report microsporidiosis (E. cuniculi, E. intestinalis) in Mosul city - Iraq.