Keywords : Phylogenetic tree


Genotyping study of Fasciola gigantica isolated from cattle in Aqrah city, Iraq

Reedha N. Hamoo; Fouad S. Al-Rubaye; Nashaat G. Mustafa

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 123-127
DOI: 10.33899/ijvs.2019.125621.1108

This study was conducted to investigate the 18S rRNA gene of Fasciola gigantica obtained from the liver of cattle live in Aqrah city, Iraq. Fifty-nine Fasciola flukes were collected through routine investigation from livers of naturally infected local cattle (42 cows), from May to August 2017, at the central slaughterhouse of Aqrah city, Kurdistan region of Iraq, the flukes were washed by PBS and then fixed in ethanol. Genomic DNA was extracted, and a 560 bp fragment was amplified by PCR, subsequent by sequencing of PCR products. A remarkable result of this project was the deposition of our gene isolate in GenBank (Accession No. MG786553). However, it was confirmed by the sequence results that isolate species was F. gigantica, and interestingly our samples sequences have alignment match of 100% with many international isolates, without genetic mutations or variations. It is concluded that molecular study could be utilized for both diagnosis and differential diagnosis of parasites with huge precise. Also, an 18S rRNA gene is a perfect fragment for molecular study and phylogenetic analysis of F. gigantica, also our samples have 100% alignment match with universal isolates.

Microscopic identification, molecular and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq

Rashaa Ateaa; Mansour Alkhaled

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 251-258
DOI: 10.33899/ijvs.2019.162882

Babesia is one of hemoprotozoan parasite transmitted by arthropod vectors which responsible for causing of Babesiosis disease in bovine worldwide. The present study was designed for microscopic identification, molecular, and phylogenetic analysis of Babesia species in buffalo from slaughter house in Al-Najaf city of Iraq. The study performed in three months of summer season (August into September 2017) and animals ages and sex were included in this study. The direct microscopic prevalence results were show highest prevalence of haemoprotozoa prevalence at Babesia sp. 45.74%. The prevalence of Babesia sp. related to animal sex, were show in male 43.48% and female was 52%, with non-significant differences. The Prevalence of Babesia sp. related to age were show 12.50%, 92.86% and 30% in young, adult and old age respectively with significant differences (P<0.05). The prevalence of Babesia sp. related to month of study were show. 28.57%, 62.50% and 42.86 in August, September and October respectively and with non-significant differences. Molecular study results were based on PCR and DNA sequencing method by phylogenetic tree analysis (MEGA 6.0) and NCBI-BLAST Homology Sequence Identity to differentiation Babesia species typing. The Babesia species prevalence results were show identified two Babesia species, high prevalence of Babesia bovis (38.30%) were closed related to NCBI-Blast Babesia bovis (HQ264126.1) with homology sequence identity 97-100% and Babesia bigemina 7.45% were closed related to NCBI-Blast Babesia bigemina (KU206291.1) with homology sequence identity 95-99%, then 43 Babesia species includes (B. bovis and B. bigemina) were submitted into NCBI-Genbank and provided accession numbers (MH503811-MH503853). In conclusion, this study concluded that Phylogenetic tree and homology sequences identity was show accurate in differentiation of Babesia species, and these species can be isolated at from local water buffalo from slaughter house in Al-Najaf city, of Iraq.

Nad1 gene analysis of Echinococcus granulosus from sheep in Aqrah city, Iraq

R.N. Hamoo; nashaat Ghalib Mustafa; S.A. Abdulraheem

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 341-345
DOI: 10.33899/ijvs.2019.162965

Echinococcus granulosus (E. granulosus) is a dog tapeworm cestoda; it is larval stage responsible to cystic echinococcosis, one of the most common and dangerous worldwide zoonotic parasitic disease. The aim of this study was the molecular identification of the local strain of E. granulosus isolated from sheep liver slaughtered in the principal abattoir of Aqrah city, Northern of Iraq during Jun-Nov. 2017. In this study, 37 sheep liver infected by E. granulosus, 12 of high DNA purity fertile (have protoscolices) cyst of them were considered. A molecular study conducted on the mitochondrial NADH dehydrogenase 1 (nad1) gene. Results demonstrated that E. granulosus isolates were sheep strain (G1) genotype, with fascinating highly corresponding 95% and 96% to global isolates, particularly to north African and Mediterranean countries, by employing phylogenetic tree analysis. So, the isolates of our project were deposited in Genbank (accession No. MG792129). This study findings provide that the local isolates of E. granulosus from sheep liver in Aqrah city, Northern of Iraq are loyally equivalent to global strains and isolates, in addition, nad1 gene considers a perfect biomarker in a molecular identification and phylogenetic study of this parasite.

The genetic relationship for Klebsiella pneumoniae isolated from human urinary tract and beef

Saba Falah Klaif; Hassan Naser; Jenan N. Sadeq

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 75-80
DOI: 10.33899/ijvs.2019.125531.1053

The present study aimed to describe the genetic relationships of zoonotic characterization of Klebsiella pneumoniae isolated from Human urinary tract and beef. The study includes (50) urine samples from human and (50) beef samples. The isolation and identification of Klebsiella pneumonia were done by using enrichment culture method and Vitek 2, then confirmed by PCR technique based on 16S ribosomal RNA gene which designed in this study using NCBI-GenBank (LT599801.1) and DNA sequencing was done on some positive isolates. The results show that Klebsiella pneumoniae was isolated from Beef at 38(76%) And from human at 32(64%) by vitek2. The PCR technique was show highly sensitive and specific confirmative detection of Klebsiella Pneumonia isolates at Clarify DNA sequencing of a partial sequence of 16S ribosomal RNA gene was shown homology sequence identity highly with NCBI-Blast Klebsiella pneumoniae isolates. The phylogenetic analysis was show clear genetic similarity at (0.5 genetic change) between human and beef in Klebsiella pneumoniae isolates. The gene sequence deposited into NCBI-GenBank accession numbers (MF314450.1, MF314451.1, MF314452.1, MF314453.1). In conclusion, the study presents the first report in Iraq of genetic relationship among K. pneumoniae isolates from beef and humans. Therefore, it is essential to define the role of animals as an important source for the distribution of pathogen related to public health.