Author : Abdullah, O.A.

Using ELISA technique for the detection of aflatoxin M1 in thick cream

S.D. AL-Sawaf; O.A. Abdullah

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 111-114
DOI: 10.33899/ijvs.2020.166882

This study involved a disclosure of Aflatoxin M1 in 50 samples of thick cream (Gaymer), 30 were manufactured from raw buffalo milk and 20 were collected from local markets of different regions in Mosul city, by using Enzyme Linked Immunosorbent Assay (ELISA) method. The results revealed that 90% of the manufactured thick cream were positive to residual AFM1, with a mean value of 16.164 ng/Kg. Only 3.3% of the manufactured thick cream samples had a concentration values above the permissible limits. Within the same subject, local thick cream samples showed a higher AFM1 level than the manufactured samples with a mean of 29.158 ng/Kg and 15% of local thick cream samples had higher AFM1 than the permissible limits according to European Commission. (i.e 50 ng /Kg).

Use of ELISA technique for detection of streptomycin residues in milk

O.A. Abdullah

Iraqi Journal of Veterinary Sciences, 2012, Volume 26, Issue Suppl. II, Pages 165-168
DOI: 10.33899/ijvs.2020.166892

This study involved examination of 45 milk samples were distributed as: 15 imported milk powder, 10 imported liquid milk, 10 local cow's milk, 10 local sheep's milk. These samples were collected from September 2010 to April 2011 at different markets in Mosul city (Iraq) in order to detect level of streptomycin residue using Enzyme Linked ImmunoSorbent Assay (ELISA) technique. The results showed that the percentage of positive samples of streptomycin residue in milk was 53.3%, and as follow 15.6% in the local sheep's milk samples, 13.3% in the local cow's milk samples, 11.1% in the imported liquid milk samples and 13.3% in the imported milk powder samples with mean value of 22.8, 20.1, 12.6 and 19.9 µg/kg milk, respectively. From these results, it is clear that all samples of milk had residual streptomycin level within the permissible limits and it is regarded as safe for human consumption.