Author : Peter, Sherly

Pathogenesis of Salmonella enterica serovar albany in experimental infected SPF BALB/c Mice

Jesny B. Sabri; Imad I. Al-Sultan; Khalil Altaif; Sherly Peter; Mohammed J. Saadh

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 339-344
DOI: 10.33899/ijvs.2019.126269.1282

Salmonellosis remains an important zoonotic disease and public health concern, Salmonella enterica serovar Albany is one of the motile serovars which has been identified from poultry and humans. However, its pathogenic potentials and shedding probability and duration from infected/colonized chickens have never been reported. To assess its pathogenic potentials and shedding probability 6 SPF BALB/c mice was inoculated with 0.1ml volume for each mice bacterial solution of 108 CFU/ml of Salmonella entrica serovar Albany after 24 hours the segments of the duodenum, jejunum, ileum, caecum and colon were fixed to study the histopathology and the polymerase chain reaction (PCR) was used to confirm the Salmonella entrica serovar Albany in the intestinal mucus swabs. The control group consist of 6 SPF BALB/c mice were inoculated with 0.1ml of 0.9% normal saline, The pathogenesis incidence rate of the disease caused by S. entrica serovar Albany revealed that prominent blood vessels on caecum 100%, red intestinal serosal 100%, infiltration of inflammatory cells in the crypt of liberkhun and submuscular layer of small intestine 100%, enterocyte necrosis 100%, haemorrhagic enteritis 83.3%, cecitis 33.3%, colonitis 66.6%, villus atrophy 100%, crypt atrophy 100%, and detachment of epithelial tissue 50%, can occur as soon as 24 hours post infection. Infected S. entrica serovar Albany was also successfully re-isolated from the intestinal swabs which revealed that the mice is potentially shed the bacteria through feces.