Author : Zanan M. Taha

Genetic diversity and clonal relatedness of Aeromonas hydrophila strains isolated from hemorrhagic septicemia’s cases in common Carp (Cyprinus carpio) farms

Zanan M. Taha

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 643-648
DOI: 10.33899/ijvs.2020.127566.1511

The objective of this study was to determine molecular typing and comparison analysis of 24 Aeromonas hydrophila isolated from the diseased fish with hemorrhagic septicemia in freshwater ponds and cage in Mosul and Duhok cities, Iraq. A total of 24 A. hydrophila isolates that were collected from various fish ponds and cage, were used in this study. Identification of isolates was made by the standard microbiological and molecular methods. ERIC-PCR was done with different primers to establish the genetic relationship between strains. ERIC-PCR typing showed that 24 strains of A. hydrophila were classified into 11 ERIC types (genotypes). Genotypes 9 and 7 represented the most prevalent clone. All A. hydrophila strains that were isolated from the same fish were genetically diverse. There was minimal genetic similarity between some strains which were retrieved from the same geographical source area. Also, some isolates from different geographic source area were showed a 100% genetically similar. Aeromonas hydrophila was genotypically heterogeneous and clonally dispersed among different fish ponds and cage in Mosul and Duhok cities, Iraq. Besides, one fish can be infected with more than one strains of A. hydrophila.

Investigation of gcat gene and antibiotic resistance pattern of Aeromonas hydrophila isolated from hemorrhagic septicemia’s cases in fish farms

Zanan M Taha; Shaaban Tayar Sadiq; Warheen Aziz Khalil; Kaheen Yusif Muhammad-Ali; Hayam Salih Yosif; Hozan Nizar Shamil

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 2, Pages 375-380
DOI: 10.33899/ijvs.2020.126876.1405

The significance of Aeromonas hydrophila concerning hemorrhagic septicemia in aquaculture farms production in Duhok province, Iraq was investigated. Antibiotic-resistant profiles of isolates were also investigated with 8 antibiotics. Bacterial isolates were identified by using morphological and biochemical tests and confirmed molecularly by amplification of gcat gene. Out of 25 examined fish, only 19 fish were harbored A. hydrophila. Twenty-four A. hydrophila strains were isolates from 100 organ samples. Ninety-six percentages of the isolates were resistant to each of the imipenem and gentamicin, followed by doxycycline 92%, ciprofloxacin and trimethoprim-sulfamethoxazole 88%, norfloxacin 58% and ceftriaxone 33%. None were resistant to levofloxacin. Eighty-eight percentages were multiple antibiotics resistant. The high isolation rate of A. hydrophila in our study indicates that this species was the major cause of the outbreak in hemorrhagic septicemia’s cases in our area affecting carp farms and the high rate of resistance should be considered as these isolates can serve as a resistance source for human being during food series and make a great challenge for their therapeutic opportunity.