Author : Jarad, Noor

Molecular detection of Cryptosporidium parvum in chicken in Al-Diwaniya province

Noor I. Jarad

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 441-445
DOI: 10.33899/ijvs.2019.126159.1249

This study were conducted in Al-Diwaniya province, in south Iraq during the period from Februaryto July 2019 to determine the rate of infection of Cryptosporidium parvum in domestic chicken, study the effect of some epidemiological factors such as sex and months on the rate of infection, addition to the molecular identification of Cryptococcus parvum by amplification HSP70 gene by conventional PCR. Number of collected fecal sample was 210 from domestic chicken and stained by Ziehl-Neelsen stain. The results of the microscopic examination showed that 108(51.4%) out of 210 fecal samples were infected with Cryptosporidium spp. The statistical analysis founded no marked difference in prevalence of infection between sexes. Significant difference was recorded between infection rate during the months of the study and higher prevalence of infection rate was observed in March 11.9%, while lowest infection rate was observed in July 5.23%. and June 5.23%. Genomic DNA was extracted from 108 fecal samples and HSP70 gene for C. parvum was amplified by PCR. PCR technique is showed that out of 108 fecal samples 21.3% were positive for Cryptosporidium parvum.

Serodiagnosis of Toxocariasis by ELISA test using anti- T. canis IgG antibodies in stray dogs compared to PCR

Noor Jarad; A.K. Abbas; N.N. Aἀiz

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 367-370
DOI: 10.33899/ijvs.2019.163081

Toxocara (T.) canis is a nematode parasite of canines; belong to the Ascarididae family, which accidentally infected humans. Puppies expel the eggs with the feces from the fourth week of the life cycle. This study is the first study in Iraq for detection seroprevalence in stray dogs and extended from January to September 2017. Our study was aimed to investigate the seroprevalence of T. canis infection in stray dogs from different areas in the Al-Diwaniya province, Iraqto detection of specific IgG antibodies to T. canis compared to Conventional PCR technique with the effect of the risk factor. One hundred of the blood sample and one hundred of a faecal sample of same dogs after shooting were studied usingindirect ELISA test and PCR. The result revealed that 71% of the dogs had a seropositive result for this parasite by ELISA test. Dog age is an important factor and affects seroprevalence, were shown that positive rate in adult dogs was more 83.05% than the young dogs 53.65%, while no significant between dogs according to sex. PCR technique showed 58% of dogs were positive forinternal transcribed spacer 1 (ITS1) ribosomal RNA. The sensitivity and specificity of ELISA test was 79 and 40% respectively.