Author : Suwarno, Suwarno

Molecular Analysis of ompA Gene Pasteurella multocida Indonesia Local Isolates

A. T. Soelih Estoepangestie; Arini Rahmi Dewi; Suwarno Suwarno; Didik Handijatno; Rahaju Ernawati; Wiwiek Tyasningsih

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2019.125934.1191

The aim of this research was to analyze ompA molecular gene of Pasteurella multocida buffalo isolate and bovine isolate from Nusa Tenggara Timur, Indonesia and Katha strain isolate from Haemorrhagic Septicemia Vaccine. Determinant of P.multocida local isolates ompA gene amplification sequencing PCR then conducted to see the sequence of nucleotide sequences of ompA gene. The results of PCR amplification showed an amplicon of 559 bp of all isolates. The homology analysis result of the isolates ranged from 93 – 100% with 13 P.multocida isolates from GeneBank, and phylogenetic tree analysis shows that buffalo isolate were closely related to Katha strains, Iran, India and China isolate. Whereas bovine isolate far enough with buffalo and Katha strain isolate. Nucleotide sequences were compared to amino acids then by the method of Kolaskar and Tongaonkar antigencity predicted antigens in P.multocida. Epitope predictions from local isolates and Katha strain were found in five peptides: QVSPVFAG, IPELALRVEYQ, GQSVYVPEVVSKT, LKSASVAVAG, and ANYLVAKG .

Detection of the extended spectrum β-lactamase produced by Escherichia coli from dairy cows by using the Vitek-2 method in Tulungagung regency, Indonesia

Akyun R. Putra; Mustofa H. Effendi; Setiawan Koesdarto; Suwarno Suwarno; Wiwiek Tyasningsih; Acts T. Estoepangestie

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 203-207
DOI: 10.33899/ijvs.2019.125707.1134

The use of antibiotics in inappropriate on food producing animals can lead to resistance many of the pathogenic bacteria to the various types of antibiotics, one of which is the Escherichia coli (E. coli) which produces extended spectrum β-lactamase (ESBL). Antibiotic resistance in animals and humans has become a global problem that needs attention and immediate management by using specific antibiotics that used for therapeutic the infected animals. The aim of this study was to isolate and detect E. coli producing ESBL. All E. coli from the surface of dairy cow rectal swabs in Sendang District, Tulungagung Regency, Indonesia using the Vitek-2 method. The number of rectal swab samples used in the present study was 50. The results of this study showed that all the samples were suspected of being E. coli, based on the morphological growth of colonies on the EMBA media. The isolates were identified by using the biochemical tests. All the samples were positive. In this study the double disc synergy test (DDST) method was using to confirm the ESBL. The antibiotics were used amoxicylyn-clavulanate, ceftazidime and cefotaxime for DDST. In additional ESBL confirmation test was used the Vitek-2 method. The presence of ESBL producing by E. coli isolated from rectal dairy swabs in tulungagung was 6% (3/50).