Author : Ali, Asmaa A.

Application of ultrasound contra infection with secondary hydatidosis in mice

Baraa B. Ramdan; Asmaa A. Ali

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 397-403
DOI: 10.33899/ijvs.2019.126132.1240

The present research explained the impact of ultrasound on the viability of Echinococcus granulosus protoscolices in vitro application of fixed frequency waves at 20000 pulse/sec and 1.8 w/cm2, with different exposing periods, thirty, twenty, fifteen, twelve, ten and five seconds respectively, subsequently prevention of mice against interjected with minor hydatid cysts, in comparison with the control animals infected with protoscolices without exposure to ultrasound waves, depending on many criteria including the numbers, weights and diameters of developed hydatid cysts. The results displayed an obvious impact of ultrasound waves on Echinococcus granulosus protoscolices viability number by increase of exposure period in vitro, in addition to a decrease of 100% of hydatid cysts numbers in the dealt ones with 17% fertility exposed for 20 seconds, the reduction rate was 99.23% in the group of fertility 35% which exposed for 15 seconds, after four and five months of infection.

Effect of probiotic acidophilus plus against infection with secondary hydatid disease in BALB /c mice

Suhayla Y. Yousif; Asmaa A. Ali

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 115-121
DOI: 10.33899/ijvs.2019.125613.1104

The current study investigated the effect of acidophilus plus probiotic in the immune activities in mice to infestation with the cystic echinococcosis. Two dilutions of the probiotic bacteria 9*106/0.1 ml, 30*103/0.1 ml CFU were used, by intraperitoneally injection in the experimental animals, pre and post infections with protoscoleces of Echinococcus granulosus. Before infection remedy comprised injection by acidophilus plus twice with 72 hours interval, on the seventh day, animals were injected with protoscoleces intraperitoneally, after infection remedy implicated injection of animals with protoscoleces of Echinococcus protoscoleces first, next to 72 hours, probiotics were inoculated intraperitoneally. Many criteria were taken into consideration including, numbers, weights, diameters and percentage reduction of hydatid cysts of treated mice in contrast to the animals infested with only. The study showed a decline in cysts including their diameter, weighting, digit, accompanied by increasing the percentage reduction of hydatid cysts in treated mice, the highest percentage reduction was 98.03%, at both dilutions, 6 months post infection, and the minimum cysts number was 0.8 in comparison with the control group 39.4, with significant difference, in the same experiment. The minimum cysts weight was 0.0104 gm, 6 months post infection at the dilution 9*106/0.1 ml CFU, compared with the control group 0.442 gm. The smallest cysts diameter was 0.057 mm in comparison with the control group 0.882 mm at dilution 9*106/0.1 ml CFU, 6 months post infection. Acidophilus plus it may well be deduced that probiotic bacteria can be used as medicinal and remedial method against infection with hydatidosis.