Author : Sorour, Sh.S.

Prevalence of nematodes infestation in Clarias gariepinus from El-Burullus Lake and Lake Nasser, Egypt

Sh.S. Sorour; A.H. Hamouda

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 181-188
DOI: 10.33899/ijvs.2019.162963

A total number of 400 Clarias gaiepinus were collected alive from several and various locations at El-Burullus Lake and Lake Nasser, to investigate the prevailing nematodes that infect this fish species from the two locations. Nearly all the examined fish were positive for one or more nematodes; four spp. of two families were identified from Clarias gaiepinus at El-Burullus Lake: Procamallanus laeviconchus, Paracamallanus cyathopharynx, Neocamallanus spp. (recovered from the gall bladder for the first time) and Terranova spp. larvae (recorded for the first time in Kafr El-Sheikh Governorate representing new locality record), one nematode from Clarias gaiepinus at Lake Nasser: 3rd stage  larvae (L3) of Contraceacum spp. (has zoonotic importance). The prevalence of Procamallanus laeviconchus, Paracamallanus cyathopharynx, Neocamallanus spp. and Terranova spp. larva were 37.5%, 44%, 0.5% and 10% respectively, meanwhile the prevalence of L3 larvae of Contraceacum spp. were 100%. This study was planned to compare between the nematodes infecting Clarias gaiepinus from the two lakes evaluating clinical signs, postmortem examinations, parasitological examinations, seasonal prevalence and histopathological investigations of infected fish with different nematodes infestation. Clarias  gaiepinus must be eviscerated as soon as possible after catching to prevent 3rd stage larvae of Contraceacum spp. in the abdominal cavity from liberating from their sheaths and attacking the fish musculature (the edible part in the fish) as well as,prevent Terranova spp. larvae in the gills from attacking another parts of the fish and thus prevent the transmission of the nematodes of zoonotic importance to the consumers.

Use molecular techniques as an alternative tool for diagnosis and characterization of Theileria equi

M.A. El-Seify; N.M. Helmy; N.M. Elhawary; Sh.S. Sorour; A.M. Soliman

Iraqi Journal of Veterinary Sciences, 2018, Volume 32, Issue 1, Pages 5-11
DOI: 10.33899/ijvs.2018.153787

The purpose of this study was to determine the prevalence of clinical, subclinical and chronic infection with the equine parasite T. equi in some Egyptian localities (Cairo and Giza governorates). A panel of 396 equine blood samples representing 141 horses, 250 donkeys and 5 mules was collected from equines during the period from April 2015 to March 2016 using microscopic examination and conventional PCR. Microscopically a twenty two (5.56%) of 396 were positive for T. equi merozoites that appeared as small rounded, pyriform shaped and maltase cross shaped merozoites. Among 8/141(5.67%) horses and 14/250 (5.60%) donkeys were found to have positive for T.equi. A one hundred blood samples (45 horses, 50 donkeys and 5 mules) selected randomly were also examined by PCR. The results of PCR showed 30/100(11/45 (24.4%) horses, 18/50 (36%) donkeys and 1/5 (20%) mule) were positive for T.equi. When the sequenced PCR amplicons (n=3) were aligned to the reference nucleotide sequences of T. equi accessed in Genbank, the horse isolate showed insertion of Thymine (T) base at position 23 and substitution of Thymine (T) base with Cytosine (C) base at position 91, while the donkey and mule isolates have no alterations when compared to the reference sequences. The phylogenetic analysis showed that the sequenced PCR isolates belonged to T. equi. The obtained sequences were deposited in the GeneBank database under accession numbers MF192854, MF192855 and MF192856.