Author : Mansour, Khalefa A.


Phylogenetic analysis of Jaagsiekte sheep retrovirus (JSRV) in Iraqi Awassi sheep

Saad Al-Husseiny; Asaad Jassim; Khalefa A. Mansour; Qassim H. kshash

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 351-355
DOI: 10.33899/ijvs.2019.126172.1255

Jaagsiekte sheep retrovirus (JSRV) infection in sheep results in contagious ovine pulmonary adenocarcinoma (OPA). The aim of this study was to detect the presence of JSRV in Iraqi Awassi sheep of Al-Qadisiyah Province using reverse transcription-PCR (RT-PCR). Lung secretion samples from 50 adult sheep (1.5 to 2-year-old) during 2016-2018 that were suffering from chronic respiratory distress with suspected clinical OPA were collected. RT-PCR assay for detection of JSRV was conducted using primers specific for its 382 bp envelope protein gene. The results showed that 22 of the 50 (44%) lung secretion samples were positive for JSRV. Phylogenetic tree analysis revealed the JSRV sequence detected in the local Iraqi sheep population was closely related to NCBI BLAST locus isolates KT279066.1 and KT279065.1. The study found that the JSRV disease is prevalent in the Iraqi Awassi sheep flocks and that the local JSRV isolate is genetically divergent from most other global isolates. 

Phylogenetic tree analysis study of bovine papillomaviruses type 1 based on L1 gene in Al-Qadisiyah governorate, Iraq

Khalefa Ali Mansour; Hassan Hachim Naser; Muthanna Hadi Hussain

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 1, Pages 151-155
DOI: 10.33899/ijvs.2019.125535.1057

Bovine fibropapilloma and papilloma occur in different parts of the skin of animals. Bovine Papillomavirus (BPV) is an oncogenic virus making benign tumor lesion of together mucosal and cutaneous tissue in cattle. In order to confirm the clinical diagnosis; the study planned to make the molecular detection of BPV (DNA) using Polymerase Chain Reaction (PCR) from skin lesions and the phylogenetic analysis. Thirty-eight samples of skin lesions were collected from cattle clinically suspected to be infected with bovine papilloma virus from herds in Al-Qadisiyah Governorate in 2016, the primary clinical diagnosis depended on the morphological appearance and features of the lesion. Deoxyribonucleic Acid (DNA) was extracted from skin lesions; the DNA was examined by PCR technique using specific primer to BPV-1 /L gene-1. Twenty-two samples out of 38 (57,9%), which were collected from different regions in Al-Qadisiyah Governorate, were positive. The sequences of four positive samples of DNA product amplification of (BPV) type-1, L1 gene confirmed the PCR results. These samples had the DNA presented in four accession numbers KY662042-1, KY662043-1, KY662040-1 and KY662041-1. This study proofed that cutaneous bovine papillomatosis related with BPV-1 infection in the cattle herds has affinity to solid skin rather than other epithelial and mucosal tissue.