Author : Hassan, Ashwaq Ahmed


Effect of vitamin C and acetylsalicylic acid supplementation on some hematological value, heat shock protein 70 concentration and growth hormone level in broiler exposed to heat stress

Ashwaq A. Hassan; Rana A. Asim

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 357-363
DOI: 10.33899/ijvs.2019.125950.1195

The goal of the current investigation is to study the effect of vitamin C and acetylsalicylic acid in minimizing the effects of heat stress in terms of hematological values, growth hormone, HSP70 and glutathione. Broiler were randomly distributed into four groups: 1st group was served as the control, the 2nd group subjected to heat stress 40±2 ºC up to 4 hours/day, the 3rd group was subjected to heat stress and vitamin C 360 mg/L via drinking water and the 4th group was exposed to heat stress and acetylsalicylic acid 0.03% via drinking water. The result showed that exposure to heat stress decline in RBCs count, Hb concentration, PCV and percentage of lymphocyte, furthermore elevation in of MCV, heterophils, H/L ratio, and Hsp70 concentration. Administration of vitamin C caused a significant rise RBCs, percentage of lymphocyte and reduces in MCV, MCH value, percentage of heterophils and H/L ratio compared with the heat stress group. Administrations of acetylsalicylic acid were significantly increased the RBCs, and PCV and decrease in MCV, MCH, MCHC values, and H/L ratio compare with the heat stress group. The results did not show a significant change between the tested groups in the levels of glutathione and growth hormone. The study concluded that Vitamin C and acetylsalicylic acid administration as feed additive ameliorating the opposing effect caused by heat stress in the broiler; thus, its administration recommends when there is heat stress exposure.

Effect of supplementation of encapsulated organic acid and essential oil Gallant+® on some physiological parameters of Japanese quails

Hiyam N. Matty; Ashwaq A. Hassan

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 181-188
DOI: 10.33899/ijvs.2019.125732.1142

The current study was conducted to investigate the effects of dietary supplements of organic acid and essential oil Gallant on the growth hormone, glutathione, performance of growth, some biochemical parameters and intestinal histomorphology in quails. Japanese quails one-day-old (n=120) were distributed randomly into four groups included 10 / 3 replicates for each group. The G1, was control group that was fed on a basal diet. Quails of G2, G3, and G4 were fed on a supplemented diet Gallant 300, 600 and 900g/ton,respectively. Administration of Gallant 600 g/ton and 900g/ton to quail led to significant decrease in triglyceride while supplementation with 600 g/ton caused decrease in cholesterol. On the other hand, 300 g/ton caused an increase in final body weight and total weight through the duration of the experiment, as well as a decrease in total feed consumption and the best feed conversion ratio in all supplementation with 300 g/ton caused a significant growth hormone elevation. All feed additives didn't affect the level of glutathione. The addition of Gallant additive groups. Interestingly, the addition of different doses of Gallant to the diet increased villus length and width, crypt depth, villus / crypt ratio, percentage of goblet cell, apparent surface area, and intestinal epithelium thickness compared to the control group. It was concluded that dietary supplementation with different doses of Gallant improved growth hormone, growth performance and intestinal histomorphology in Japanese quails, and dietary supplementation with organic acid and essential oil as alternatives to the growth promoter of antibiotics.

The relationship between HSP70 and level of leptin and luteinizing hormones in female rats exposed to chronic and acute heat stress

Hiyam N. Matty; Ashwaq A. Hassan

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 37-43
DOI: 10.33899/ijvs.2019.125565.1082

The current search experiments designed to study the effect of chronic and acute heat stress on the relationship between HSP70, leptin and luteinizing hormones level in female rats. 45 female pups were used in the age of weaning, were randomly divided into three equal groups. 1st group was a control group, 2nd group exposed to chronic heat stress at 38 ºC for 1hour a day since the age of weaning until onset of puberty and the 3rd group exposed to acute heat stress at 38 ºC for 4 hours per day for 5 consecutive days from 35 day age of rat and each group above were secondary divided into 3 age groups consisted of pre-puberty, at puberty and post-puberty. Results of study showed female rats exposed to chronic and acute stress led to significant increase in the level of luteinizing hormone at onset of the puberty. The result showed correlation coefficient between the level of HSP70 and leptin hormone significantly in the females acute stressed group at onset of puberty, as well as female exposed to chronic heat stress led to significant correlation coefficient between HSP70 and leptin hormone at post puberty. The result revealed significant correlation coefficient between HSP70 and luteinizing hormone in female control group at onset of puberty. It concluded from this study there is relationship between HSP70 and leptin hormone at onset puberty during acute heat stress and same relationship at post puberty during chronic heat stress.

Effect of heat stress on sex hormones, sex organ weight and relationships with sexual puberty in male rats

hiyam matty; Ashwaq Hassan

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 63-71
DOI: 10.33899/ijvs.2019.163089

This study was designed to investigate the effect of chronic and acute heat stress on sex hormones level and their relationship to sexual puberty in male rats. Used 45 male pups in the age of weaning, were randomly divided for 3 groups each group included 15 pups of each sex. 1st group was a control group, 2nd group exposed to chronic heat stress at 38Cº for 1hour a day since the age of weaning until onset of puberty and the 3rd group exposed to acute heat stress at 38Cº for 4 hours per day for 5 consecutive days from 35 day age of rat and each group above were secondarily divided into 3 age groups consisted of pre-puberty, at puberty and post-puberty. Results of study showed that male rats exposed chronic heat stress led to a significant increase in body weight, tail of epididymis, prostate gland weights, at onset of puberty compared with control group, with a significant decrease in body weight, testis, head and tail of epididymis, prostate and seminal vesicle weights at post-puberty compared with control group. as well as a significant decrease in the level of luteinizing hormone at post-puberty compared with acute heat stress group. Males exposed to acute heat stress cause a significant decrease in the head and tail of the epididymis, prostate, and seminal vesicle weights and significant increase in the concentration each of testosterone and luteinizing hormones at post-puberty compared with the control group. Acute exposure to heat stress caused early sexual puberty in males to show from the early appearance of first sperm compared with the control group, while the exposure to chronic heat stress led to delay of puberty associated with a delay in the appearance of first sperm compared with the control group. It concluded from this study that male rat's exposure to heat stress affects differently on sex hormone. Acute heat stress led to reach early sexual puberty and reflected the impact on puberty when chronic exposure to heat stress.

Assessment the level of some heavy metal in vital body fluids and blood parameters in lactating Awassi ewes

Ashwaq Ahmed Hassan; Rana amer asim Al Suleman agha; Ali Saeed Al-Chalabi

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 297-301
DOI: 10.33899/ijvs.2019.162908

The aim of the study was to estimate the level of cadmium, zinc and oxidative stress parameters in serum and milk as well as the blood constituents in local at the age of 3-4 years during the spring and summer seasons. Blood and milk samples were collected from 30 ewes (15 each season). The blood samples were allocated to into two tubes, for complete blood profile study and for biochemical analysis. Milk was used for biochemical tests. Blood analysis showed a significant difference between some blood values during the two seasons represented by significant increase of total white blood number, haemoglobin concentration and packed cell volume in circulating blood of lactating ewes during summer season compared with spring season. Red blood cells number and mean corpuscular haemoglobin concentration did not change among the two seasons. Both mean corpuscular volume and mean corpuscular haemoglobin value of lactating ewe increased significantly during spring season with respect to the summer season. The serum glutathione level showed a significant elevation during spring season compared with the summer season, while, the level of malonaldehyde and peroxynitrite free radicle remain constant during the study period. There were no significant changes in the level of cadmium in serum and milk of examined ewes, while the serum level of zinc significantly higher during the spring compared to the summer season and milk zinc level remain stable during both seasons. The study concluded variations in haematological, oxidative stress biomarkers, serum and milk concentration of Zn and Cd according to lactation season in ewes.