Author : Alabdaly, yamama zuher

Toxic effects of butylated hydroxytoluene in rats

Yamama Z. Alabdaly; Entisar Kh. Al-Hamdany; Entisar R. Abed

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 121-128
DOI: 10.33899/ijvs.2020.126435.1322

This study aimed to assess the acute toxicity in rats of heated and un heatedbutylated hydroxytoluene (BHT). Sunflower oil dissolved BHT, heated at 98±2ºC by a water bath. The animals were divided into five groups. The control group dosage orally with sunflower oil, the first group treated with 250 mg/kg BHT, the second group treated with 250 mg/kg heated, BHT the third group treated with 500 mg/kg BHT and the fourth group treated with 500 mg/kg heated BHT. All groups received oral treatment. The results showed a substantial reduction in motor activity relative to other groups at a dose of 250 mg/kg heated BHT. There was a substantial distinction in the negative geotaxis test in groups of 500 mg/kg heated and un-heated BHT, while a cliff avoidance test in the heat treated dose of 250 and 500 mg/kg was observed in the cliff avoidance test compared to other groups. A significant reduction occurred in all groups in the pocketing and dorsal tonic immobility test. The pathological changes of heated BHT groups were more severe than those of un-heated BHT groups especially the dose of 500 mg/kg heated BHT. It represented by coagulative necrosis, muscle atrophy in heart, interstitial pneumonia, serofibrinous exudate, pulmonary emphysema in lung and neuronal degeneration, microgliosis, myelin vacuolation and satellitosis in the brain. The study concluded that heated BHT at a dose of 250 and 500 mg/kg had toxic effects to motor and neurobehavioral activity, and histopathological changes in the brain, heart, and lung.

Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats

yamama zuher Alabdaly; Mohammed Ghassan saeed; Hadeel mohammed Al-hashemi

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 151-156
DOI: 10.33899/ijvs.2020.126490.1335

This study aims to investigate the effect of aspirin in methotrexate toxicity (increase or decrease) relating to oxidative stress and histopathological changes of the liver and kidney in male rats. Twenty rats were divided into equal 4 groups, the first is considered control group, the second was treated with methotrexate in a dose of 10 mg/kg, the third was treated with methotrexate and aspirin in doses of 10 and 30 mg/kg respectively, the fourth was treated with aspirin alone with a dose of 30 mg/kg. All doses were given by daily oral dosage for 4 constitutive days. The result revealed a significant decrease in the concentration of both glutathione and albumin and a significant increase in the concentration of both malondialdehyde and ALT enzyme in the two groups treated with methotrexate alone or in combination with aspirin as compared to the control group. The histopathology revealed that the severity of lesions was in the group of methotrexate with aspirin, group of methotrexate only and a group of aspirin respectively, which are representing by coagulative necrosis and hypertrophy of hepatocytes in the liver while the lesions of kidney were atrophy of some glomeruli and renal cystic formation. The study concludes that aspirin increases the toxic effect of methotrexate at the level of oxidative stress concomitant with the occurrence of hepatic and renal toxicity.