Author : Alobaidii, Wasan Amjad

Molecular detection of Hammondia heydorni in dogs in Mosul city

Wasan A. Alobaidii

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 329-332
DOI: 10.33899/ijvs.2019.126052.1219

Through this study 122 individual fecal samples were collected from dogs, with different ages, sexes, and breeding management (stray and pet house hold). Some dogs were with healthy appearances while others were suffered from diarrhea, anorexia, depression, dehydration. Infection was investigated by light microscope after flotation technique by using Sheather's sugar solution. if the sample gives positive to oocyst (H. heydorni or other relative group), were mixed with potassium dichromate solution a rate of 2.5% for the stimulation of sporulation. DNA extraction was done using 2% sarcosyl, pronase E followed by phenol/chloroform extraction then precipitation attempt using ethanol. DNA amplification was attempt using H. heydorni primers JS4 and JS5 n microscope examination give 78 sample positives to the presence of oocyst, while polymerase chain reaction showed total percentage of infection with H. heydorni was 34.6% (27 case give positive reaction to PCR). Dogs less than 6 months of age show high infective rate 17.9%, dogs which suffer from gastrointestinal tract troubles (diarrhea, anorexia and dehydration) gave high infective rate 24.4%. Stray dogs gave high prevalence of infection to H. heydorni 25.6% when compared with pet house hold dogs which recorded 9% percentage of infection. This study is the first recorded H. heydorni in dogs in Mosul city.

The serological diagnosis of canine Leishmaniasis by using ELISA in Nineveh province

Wasan Amjad Alobaidii

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 111-114
DOI: 10.33899/ijvs.2019.163194

This study was to investigate the specific antibodies of Leishmania using Enzyme-Linked Immunosorbent Assay to detect the specific antibodies of the Leishmania donovani complex (L.infatum, L.chagasi, L. donovani) in 80 dogs of both types (domestic and stray dogs) of different ages and both sexes in the Nineveh Province for the period from May 2018 to October 2018. Some animals suffered from the appearance of skin ulcers in the face and quarters and diarrhea, vomiting, Paw pad fissures, the other animals did not show any clinical finding. The results showed that the total infection rate was 55%, the highest percentage of dogs with skin lesions 80.9% with significant differences with other groups, the stray dog's groups recorded high infective rate when compared with domestic dog group 62.96 and 38.4% respectively with significant differences.