Author : Jwher, Dhyaa Mohammad Taher

A study of the gastrointestinal parasites in awassi sheep and surrounding environment

Dhyaa M. Jwher; Maan T. Jarjees; Aqeel M. Alshater

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 3, Pages 561-567
DOI: 10.33899/ijvs.2020.127174.1478

The aim of this study was to investigate the occurrence gastrointestinal parasites in Awassi sheep and the contamination of surrounding environment in ten different locations of Nineveh governorate, during March up to June/2018. A total of 781 of different samples including 231 fecal, 192 soil, 188 feed and 170 water samples were investigated for the detection of ova and oocysts. Traditional parasitic techniques were followed in the study. The results showed that fecal samples were positive for eggs of nematodes, trematodes, and protozoal oocysts 30.63, 9.09, 14.28% respectively. Soil samples were positive for eggs of nematodes, trematodes and protozoal oocysts at 21.35, 10.93, 44.79% respectively. Feed samples declared that contamination with nematodes, trematodes, and protozoal oocysts were 22.34, 26.06, 51.59%, respectively. Examination of water troughs examined, showed that nematodes, trematodes and protozoal oocysts were occurred at 14.11, 8.82, 31.76%, respectively. It concluded that parasitic infection in sheep could attain from different sources, and every effort should be applied to reduce the contamination.

Molecular characterization of extended spectrum cephalosporin resistant Escherichia coli isolated from dogs

Ihsan M. Ahmed; Sumaya Y. Aldabbagh; Dhyaa M. Jwher

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 3, Pages 473-478
DOI: 10.33899/ijvs.2020.127032.1441

Shepherd dogs have been implemented in the transmission and distribution of many threatening pathogens. The presence of extended-spectrum-cephalosporin resistant Escherichia coli (ESCR E. coli) in dog feces can constitute a significance risk to human health due to transmission of antibiotics resistance from dogs to humans, other animals and the surrounding environment. Therefore, in this study, phenotypic and molecular characterization of fecal ESCR E. coli were investigated in shepherd dogs accompanied sheepherders in urban areas. Sixty-seven fresh fecal samples were collected from shepherd dogs from different regions of Mosul city. Bacteriological examination of ESCR E. coli was done using MacConkey agar with cefotaxime followed by subsequent PCR confirmation of the CTX-M gene using specific primers and molecular characterization using specific primers directed to CTX-M-1, 2 and 9 groups. The results of bacterial examination showed successful confirmation of ESCR E. coli which has been isolated from fecal samples of shepherd dogs 58.2% (39/67). In addition, detection of CTX-M gene was confirmed in 53.7% (36/67) of E. coli isolates. Furthermore, molecular characterization of CTX-M gene revealed the presence of only one genotype belongs to CTX-M-1. However, both of CTX-M-2 and CTX-M-9 genotypes were not detected in this study. This study concluded that shepherd dogs have an essential role in carrying and spreading of ESCR E. coli especially in urban regions.

Detection of multiple presence of antibiotic residues in slaughtered sheep at Duhok abattoir, Iraq

Shireen A. Yousif; Dhyaa Mohammad Taher Jwher

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 49-55
DOI: 10.33899/ijvs.2019.126259.1276

The aim of this study is to investigate the presence and/or multi-presence of tetracycline, penicillin G, streptomycin, and gentamicin residues in slaughtered sheep carcasses at Duhok abattoir using ELISA technique. Eighty-eight samples were collected from different sites of twenty-two sheep carcasses. These samples included Longissimus dorsi, diaphragmatic muscles, liver and kidney. The samples were prepared, homogenized, extracted and assayed according to ELISA kit instructions. The results revealed that all 22 carcasses were contained at least one type of tested antibiotics. Multiple antibiotic residues were found in 17(77.27%) of examined carcasses. The study indicated that local ovine meat sold in Duhok province generally contained residues of antibiotics. Animals slaughtered might have been treated with veterinary drugs and the proper withdrawal period was not respected before slaughtering.

Detection of similarity and genetic distance between Iraqi chicken varieties and different standard strains

Thamer A. Ezzulddin; Dhyaa M. Jwher; Sufian A. Dabdoub

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 333-337
DOI: 10.33899/ijvs.2019.126109.1235

Forty-eight wing vein blood samples were collected from different locations of poultry rearing farms and back yard chickens of Nineveh governorate from the of local and exotic chicken. The chicken divided into twelve groups four birds each according to colors and phenotype for the local and exotic chicken respectively. Blood DNA was extracted and amplified by thermocycler apparatus and the electrophoresis was done using 1.2% agarose gel for DNA bands exhibiting. The results showed high genetic similarity within the local chickens ranged between 0.78- 0.96 at an average of 0.88, while it ranged between 0.73- 0.86 at an average of 0.78 in exotic breeds. The degree of similarity between Iraqi and exotic breeds was 0.74-0.88 at average of 0.80. The calculated average of differences among each of Iraqi and exotic chickens and in between were 0.12, 0.22 and 0.20, respectively. However, the genetic distance within the local chicken, exotic breed and in between them was 0.128, 0.24 and 0.21 respectively. The study concluded that the genetic similarity was higher within local chicken groups than those of exotic breeds.