Author : Al Suleman agha, Rana amer asim

Effect of vitamin C and acetylsalicylic acid supplementation on some hematological value, heat shock protein 70 concentration and growth hormone level in broiler exposed to heat stress

Ashwaq A. Hassan; Rana A. Asim

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 357-363
DOI: 10.33899/ijvs.2019.125950.1195

The goal of the current investigation is to study the effect of vitamin C and acetylsalicylic acid in minimizing the effects of heat stress in terms of hematological values, growth hormone, HSP70 and glutathione. Broiler were randomly distributed into four groups: 1st group was served as the control, the 2nd group subjected to heat stress 40±2 ºC up to 4 hours/day, the 3rd group was subjected to heat stress and vitamin C 360 mg/L via drinking water and the 4th group was exposed to heat stress and acetylsalicylic acid 0.03% via drinking water. The result showed that exposure to heat stress decline in RBCs count, Hb concentration, PCV and percentage of lymphocyte, furthermore elevation in of MCV, heterophils, H/L ratio, and Hsp70 concentration. Administration of vitamin C caused a significant rise RBCs, percentage of lymphocyte and reduces in MCV, MCH value, percentage of heterophils and H/L ratio compared with the heat stress group. Administrations of acetylsalicylic acid were significantly increased the RBCs, and PCV and decrease in MCV, MCH, MCHC values, and H/L ratio compare with the heat stress group. The results did not show a significant change between the tested groups in the levels of glutathione and growth hormone. The study concluded that Vitamin C and acetylsalicylic acid administration as feed additive ameliorating the opposing effect caused by heat stress in the broiler; thus, its administration recommends when there is heat stress exposure.

Assessment the level of some heavy metal in vital body fluids and blood parameters in lactating Awassi ewes

Ashwaq Ahmed Hassan; Rana amer asim Al Suleman agha; Ali Saeed Al-Chalabi

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 297-301
DOI: 10.33899/ijvs.2019.162908

The aim of the study was to estimate the level of cadmium, zinc and oxidative stress parameters in serum and milk as well as the blood constituents in local at the age of 3-4 years during the spring and summer seasons. Blood and milk samples were collected from 30 ewes (15 each season). The blood samples were allocated to into two tubes, for complete blood profile study and for biochemical analysis. Milk was used for biochemical tests. Blood analysis showed a significant difference between some blood values during the two seasons represented by significant increase of total white blood number, haemoglobin concentration and packed cell volume in circulating blood of lactating ewes during summer season compared with spring season. Red blood cells number and mean corpuscular haemoglobin concentration did not change among the two seasons. Both mean corpuscular volume and mean corpuscular haemoglobin value of lactating ewe increased significantly during spring season with respect to the summer season. The serum glutathione level showed a significant elevation during spring season compared with the summer season, while, the level of malonaldehyde and peroxynitrite free radicle remain constant during the study period. There were no significant changes in the level of cadmium in serum and milk of examined ewes, while the serum level of zinc significantly higher during the spring compared to the summer season and milk zinc level remain stable during both seasons. The study concluded variations in haematological, oxidative stress biomarkers, serum and milk concentration of Zn and Cd according to lactation season in ewes.