Author : Hana Kh. Ismail

Amelioration activity of vitamin E in rabbits with brain histological alteration induced by zinc-oxide nanoparticles

Atheer Nabeel Taha; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2022.133599.2265

The Zinc-oxide in the form of nanoparticles suggested to be one of the crucial nanoparticles due to its expanse implementation in many industries, like electrons, food supplements, and maquillage and makeup this led to more exposure of the individual to ZnO NPs through inspiration, and skin penetration. This study object or designed to estimate the toxic impact of ZnO NPs on cerebral cortex, hippocampus, and cerebellum in male rabbits by studying the gross and histological changes by using the light microscope. 24 adult male rabbits divided randomly to 4 groups, each of them comprising six animals. Control group left without treatment; second group treated with 100 mg / kg B.W. of vitamin E orally, third group treated intraperitonially with ZnO NPs 600mg/kg B.W., Fourth group treated with I/P 600mg/kg B.W. of ZnO NPs in addition to 100mg/kg B.W orally of vit. E twice weekly for twenty-one days. The histological results showed degenerative, necrotic with vascular and inflammatory response in cerebral cortex, hippocampus and cerebellum in 2nd group of rabbits treated with ZnO NPs while the treated rabbits with ZnO NPs and vitamin E revealed slight improvement in histological picture of brain layers. Also, there was alteration in the level of acetylcholine in all groups in comparing with control group.

The impact of Nano Zinc oxide particles on the histology of male reproductive system of adult male rabbits

Atheer Nabeel Taha; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2022.133632.2270

The goal of our research is evaluating histological impact of nano ZnO particles (NZnO) on the testis and epididymis of adult male rabbits treated with intraperitoneal of 600mg/kg B.W. of nano ZnO particles twice a week for six weeks and illustrate the protective effect of vitamin E versus the effect of nano zinc oxide particles. Twenty-four adult male rabbits have been used in this research divided to four groups, control group injected intraperitoneal distal water, second group injected with 600mg/kg B.W of nano Zno particles intraperitoneal twice a week for three weeks, third group injected 600 mg / kg B.W. of nano Zno particles intraperitoneal and co-administrated with 100 mg / kg B.W. of vitamin E orally, while fourth group received 100mg/kg B.W of vitamin E orally. The histological results showed that the nano ZnO particles treatment cause obvious changes in testis and epididymis characterized by thickening of tunica albuginea of testis, degenerative and necrotic changes of germ cells lining the seminiferous tubules, arrest of spermatogenesis, giant cell formation also observed, degeneration and necrosis of epithelial cells lining epididymis canals, the canals are free from sperms is observed. As for the group of animals treated with nano ZnO particles co-administrated with vitamin E showed improvement in the histological changes compared with control group and group treated with vitamin E only which showed normal architectural of testis and epididymis, moreover there is decreased in the level concentration of testosterone of the animals treated with nano ZnO particles compared with other groups.

Histopathological and histochemical study of mastitis in sheep

Asseel M. Rahawi; Hadil B. Al-Sabaawy; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 3, Pages 719-723
DOI: 10.33899/ijvs.2022.131595.1978

Ovine mastitis is considered one of the problems that cause economic losses through its impact on milk production, which is unfit for human consumption. The current study aimed to shed light on mastitis in sheep in different areas of Mosul city. The results of the recent study showed the presence of mammary macroscopic and microscopic changes. Macroscopic changes were represented by the large size of the udder and changes in the color and texture, as well as clarity of sloughing and desquamation of the skin with clear exudation, especially in advanced cases. On the other hand, other samples showed the presence of hard nodules on the udders. In contrast, the histological changes represented by degenerative and necrotic changes 3.5%, and 21.4% of the sample appeared to suffer from liquefactive necrosis and abscesses infiltrated with inflammatory cells, 25% of the samples infected with granulomatous mastitis (nodular mastitis), other models showed that the udder infected with atrophy and metaplasia in the rate of 21%, thickening of the blood vessel wall with clot represented 14.2% and the deposition of calcium salt was 14.2%. The current study concludes that mastitis in sheep is widespread in sheep raised in different areas of Mosul city.

Efficacy of Urtiea dicica extract for amelioration of brain lesions induced by ethylene glycol in male rabbits

Ahmed M.A. Alsaidya; Hana Kh. Ismail; Enas S. Mostafa

Iraqi Journal of Veterinary Sciences, 2022, Volume 36, Issue 2, Pages 485-488
DOI: 10.33899/ijvs.2021.130563.1848

The nervous system is the most delicate organ to neurotoxic substances that affects the development of neurochemicals, including neurotransmitters, and organization of ion channels. Urtica dioica (Nettle) is one of the medicinal plants, which has been increasingly highlighted used for its anti-inflammatory and anti-oxidative components. This study aimed to evaluate the improvement effects of Urtica dioica extract on the histopathological lesions of nervous system in rabbit model. Fifteen male rabbits of 700-800 grams separated into three groups, the first group is the control, and the second group were treated with 0.75% ethylene glycol (EG) in water for 30 days, group three were combination treated group with both EG and Urtica dioica at 100 mg/kg for 10 days. Microscopic histological examination discovered generalized congestion of blood vessels at the cerebrum and cerebellum, degeneration and necrosis of the neuronal cells of the cerebral cortex which characterized by swelling of the degenerative cells, while necrotic cells characterized by shrunken and pyknosis of the cells in the cerebellum cortex presents of inflammatory cells in the meninges. In conclusion, the histopathological examination of brain sections of rabbits treated with ethylene glycol in addition to Urtica dioica extract showed slight improvement in the histological pictures of the cerebellum and cerebrum.

Comparison study between the efficacy of immune complex and conventionally live vaccine against Gumboro disease in broilers

Fanar A. Isihak; Hana Kh. Ismail; Abdulwahid A. Wahid

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 4, Pages 627-632
DOI: 10.33899/ijvs.2020.127366.1499

This study aimed to evaluate the immune response and histological changes of two Gumboro disease vaccines. Two hundred, days old broilers were divided into 4 groups: group A was vaccinated with live attenuated infectious bursal disease (IBD) vaccine at 7 and 21 days of age, group B was vaccinated with Immune-Complex vaccine (Icx) at 1 day old, then all broilers of the groups A, B and C were vaccinated with ND vaccine at 10 and 24 days, while the group D was negative control. The blood was collected at 1, 7,14,21,28 and 35 days of age to obtain serum for ELISA. Samples of bursa from broilers of all groups at 14 and 28 days of age were submitted for histological examination. As a result of vaccination in group A the antibody titers are elevated after the 1st and 2nd dose of vaccination at 7 and 21 days. In group B this titer is increased from 21 day of age and reaches to peak at 35 day 7810±858 with significant difference, while in unvaccinated groups C and D the titer decreased gradually. The histological examination of bursal sections in group A and B varied at 14 and 28 days post vaccination and showed degeneration and necrosis of follicular lymphocytes compared with group and D. Thus we conclude that (Icx) vaccine improves the immune response after IBD and ND vaccination in comparison with live IBD vaccine. 

Experimental study on the effect of toxin fractions isolated from hydatid cyst fluid of sheep on the cardiac muscles of mice

Hana Kh. Ismail; Ilham A. AL-Saleem; Afrah Y. Jasim

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 3, Pages 523-528
DOI: 10.33899/ijvs.2020.127124.1463

The aim of this study was to investigate the effectiveness of hydatid cyst toxin fractions in mice. Fifty male mice were divided into five groups with 10 mice for each group the first group as control the second group was injected with toxin fractions at the concentrations 25Mg/ml PBS, the third group are the mice that injected intraperitoneally with toxin fractions at the concentrations 25Mg/ml PBS and treated with vitamin E at the concentration of 40 mg/ 100 g of feed, the fourth group was injected intraperitoneally with 1 ml of raw fluid and the fifth group was injected intraperitoneally of 1ml of row fluid with the vitamin E at the concentration of 40 mg /100gm feed. The mice were sacrificed after 15 and 30days post injection, specimen of heart are fixed in 10% neutral buffered formalin for histological techniques. The histopathological changes in cardiomyocyte were edema, infiltration of mononuclear cell and thickening of blood vessels wall with congestion in it. The results confirm that the toxin fraction have more effect than the raw fluid and that there is the regeneration effect of vitamin E on hydatid cyst cardiomyocyte.

Histopathological alterations of male and female reproductive systems induced by alloxan in rats

Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 2, Pages 223-226
DOI: 10.33899/ijvs.2020.126626.1351

The objective of this study is to determine the histological effects experimentally induced by injection of alloxan 100 mg/kg B.w. on the histopathological structure of reproductive organs of male and female albino rats. The results showed that treatment with alloxan cause alteration in testis include irregular shape and size of seminiferous tubules, irregular division of spermatid cells, degeneration and necrosis of Sertoli cells and paucity of sperms in the lumen of tubules. While histological examination of epididymis showed the lumen of it free from sperms, thickening of muscular layer and interstitial tissue between the epididymis canal. The histological alteration of female reproductive organs includes disturbances in development of primary follicles of ovaries, hemorrhage in the interstitial tissue as well as atrophy in the uterine glands with hyperplasia of the epithelial cells of uterus. The conclusion of this study showed that alloxan cause histological alteration in reproductive organs of male and female rats.

Histopathological effects of experimental exposure to lead on nervous system in albino female rats

Asrar Al-khafaf; Hana Kh. Ismail; Ahmed M.A. Alsaidya

Iraqi Journal of Veterinary Sciences, 2021, Volume 35, Issue 1, Pages 45-48
DOI: 10.33899/ijvs.2019.126248.1273

Lead toxicity is a common health issue. Lead (Pb) is harmful to vital organs of body particularly the nervous system. This study aimed to estimate the effects of lead on the cerebellum, cerebrum and spinal cord in rat model, focusing on histopathological changes. 24 female mature albino rats of 200-300g randomly divided into 2 groups, the first is the control, and the second group were treated with lead acetate at dose 30mg/kg B.W. for 30 days. Microscopic examination revealed degeneration and necrosis of Purkinje cells and molecular cells and decrease in the number of granular cells and molecular cells also observed. Some Purkinje cells lost axons and shrunken and some areas showed depletion of Purkinje cells. Congestion of blood vessels with perivascular cuffing of mononuclear inflammatory cells, hemorrhage, neurophagia, glial nodules were observed in the brain parenchyma. Demyelination reported in white matter, with microglial proliferation around vertebral canal of spinal cord. This study referred to the increased risk of central nervous system damage due to the exposure to lead.

Effect of silver nanoparticles on some blood parameters in rats

Azhar AL-Baker; A.A. AlKshab; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 2, Pages 389-395
DOI: 10.33899/ijvs.2020.165812

The present study aims are investigating the effects of oral administration of AgNPs on some blood the parameters to show potential of toxicity of AgNPs as tool therapy and diagnosis. In this study, 20 healthy male and female rats were used. Randomly, rats were divided into 5 groups depending on the period variable respectively; AgNPs-treated rats' groups for a period of 3 days in concentration 0.2 and 0.4 gm/kg B.W, while the second period after 20 and 30 days after treatment with concentration 0.08 and 0.008 gm/ kg B.W. In addition to a control group. A Manual method was used to measure Hematological parameters. Rats treated with AgNPs showed the significant P<0.05 decrease in Hemoglobin, Packed Cell Volume, Red Blood Cells. Also, Mean Corpuscular Volume; Mean Corpuscular Hb and Mean Corpuscular Hb Concentration. Platelets levels and White Blood Cell Count were After the administration of AgNPs, a protective effect on hematopoietic levels and on Red Blood Cell morphology were shown respectively as it is assessed by Leishman stained blood smears resulted in ovalocytosis at the dose 0.4 gm/kg body weight, while at the dose, 0.08 gm/kg showed echinocytes, acanthocytes, tear drop, stomatocyte and codocyte (target RBCs). Thus, it is concluded that oral administration of AgNPs caused a decreased in the level of Hb concentration, PCV, RBC, MCV, MCH, MCHC, but increase PLTS and WBCs. It means that AgNPs has a toxic effect on blood.

Protective effect of placental mesenchymal stem cells on histological changes of pancreas experimentally induced by alloxane in mice

Hana Kh. Ismail; Rasha A. Al-Sabawy; Hamad J. Jumaa

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 1-8
DOI: 10.33899/ijvs.2020.163563

In an attempt to find a treatment for diabetes by Placental mesenchymal stem cells, we induced diabetes in albino mice by injected them with the diabetic agent Alloxan intra peritoneal cavity dose 70 mg / kg of body weight for the group 1 and 2, then injection of the diabetic mice with Placental mesenchymal stem cells in the peritoneal cavity for the group 2 then the glucose levels in the blood of these mice were measured by using a GLUCOTREND 2, while group 3 was control injected with normal saline only. Pancreas tissue where tested by studying histopathological effect of Alloxan, the endocrine pancreas histologically showed decreased in the size and number of Langerhans islets with vacuolar degeneration and necrosis of almost all cells in the atrophied islets. Also, there was congestion of blood vessels in the interlobular space and there was distortion of exocrine pancreas. There was vacuolar degeneration and necrosis of the epithelial cells of the acini, also there was atrophy of acini which lead to edema and dilatation of interacini space. Moreover, there was thickening in the wall of blood vessels, and there was thrombus in some blood vessels. In addition to the infiltration of inflammatory cells in the interlobular septa. Compared with the section of pancreas of control group showed the normal structure of islets notice the normal cells shape surrounding by exocrine pancreas. Then the pancreas studied after injection of Placental mesenchymal stem cells, while there was regeneration of islets and acini which seem to be normal comparing with control group, there was improvement of normal histological appearance of mice treated with alloxan and then injected with placental mesenchymal stem cells. The results of the diabetic mice which treated with placental mesenchymal stem cells demonstrated present of significant differences (P≤0.05). Our conclusion of this study revealed that placental mesenchymal stem cells have a protective role against the histological changes of pancreas induced by alloxan.

Diagnosis and histopathological study of avian influenza virus-H5 (AIV-H5) in broiler farms

Fanar A. Isihak; Hana Kh. Ismail; Abed Alwaheed A. Wahid

Iraqi Journal of Veterinary Sciences, 2020, Volume 34, Issue 1, Pages 101-107
DOI: 10.33899/ijvs.2019.125646.1120

This study was conducted for diagnosis and description of the pathological changes of AIV-H5 as the causative pathogen in Iraqi broiler farms. The current study was carried out on 84 broiler farms. Infected birds were tested for detection of the AIV infection from the tracheal swabs by rapid chromatographic AIV type A and H5 test kits. In RRT-PCR 8 samples (8 farms) of Trachea were selected to be tested by this assay. Samples of trachea, lung, and spleen from the dead birds with natural AIV-H5 infection were submitted for histopathological examination. seventy-two out of 84 farms tested for AIV-Type A gave positive results, and 58 out of 72 positives for type A-AIV gave a positive result for H5 antigen in a rapid chromatographic strip. The main gross lesions in the trachea of infected birds were severe congestion and hemorrhage. In the RRT-PCR assay, 8 out of 8 samples gave a distinct positive result for this test. The microscopic histopathological examination of infected tracheas showed obvious desquamation of lining epithelium with complete loss of cilia associated with congestion of blood vessels in lamina properia. Infected lungs revealed diffuse alveolar damage and severe multifocal vascular congestion. There was deposition of fibrinous material in the splenic tissue associated with the disappearance of the germinal centers. Thus, we concluded that AIV-H5 infection causes severe pathological and histopathological changes as a result of systemic infection. The RRT-PCR assay was highly sensitive and specific for the detection of highly pathogenic avian influenza virus subtypes.

Experimental detection of antioxidant and atherogenic effects of grapes seeds extracts in rabbits

Shihaab Al-Bajari; Moataz Al- Akash; Hana Kh. Ismail

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 243-249
DOI: 10.33899/ijvs.2019.162881

The aim of this study was to determine the ability of Grapes seeds (Vitis vinifera) aqueous, proteinous, non- proteinous and alcoholic extracts as antioxidant and antiatherogenic in rabbits after exposure to 0.5% H2O2 in drinking water for 30 days. The results illustrated capability of 0.5% H2O2 to initiate oxidative stress via significant decrease in Glutathione (GSH), glutathione peroxdiase (GPx), Catalase (CAT) and superoxide dismutase (SOD) level in heart and liver concomitant with significant increase in malondialhyde (MDA) in addition to decrease in high density lipoprotein- cholesterol (HDL-c) and increase low density lipoprotein- cholesterol (LDL-c) in serum of treated only with H2O2 rabbits. While rabbits treated with H2O2 associated with Grapes seeds (aqueous, proteinous, non- proteinous and alcohol) extracts for 15 days showed a significant increase of Glutathione (GSH), glutathione peroxdiase (GPx), superoxide dismutase (SOD) concentration in the heart and liver tissue as well as increase HDL-c, decrease in LDL-c and VLDL-c. While, the histolopathological examination of heart and liver tissue demonstrated that H2O2 induces, degeneration, necrosis and few inflammation foci in the liver and myocardial muscle. While the administration of (Vitis vinifera) aqueous, proteinous, non-proteinous and alcoholic extracts cause improvement in the histological picture of the liver and the myocardial muscle. It was concluded that the (Grapes extracts) aqueous, proteinous, non-proteinous and especially the alcoholic extracts can reduce the damage caused by H2O2 and this will pave the way to investigate the protective effects of the natural substances in the diseases caused by oxidative stress.