Author : Al-Attar, Mozahim yasen

Isolation and identification of Circovirus in pigeon

Safwan Yousif Al-Baroodi; Mozahim yasen Al-Attar

Iraqi Journal of Veterinary Sciences, In Press
DOI: 10.33899/ijvs.2020.126706.1364

The purpose of this study is first trial to detect of pigeon circovirus, so 1sr group include 100 cloacal swabs were collected 55 healthy and 45 ill pigeons, 36 yearlings and 64 adults, the 2nd group included organs was liver, spleen, bursa of Fabricius from 41 young pigeons 10-30 days old and bursa of Fabricius, liver, spleen from 28 dead in shell pigeon embryo in the 3rd group. DNA extracted from this samples and detection of virus DNA was attempt using polymerase chain reaction, after DNA amplification, the final products of the amplicon with 331 bp was cleared by using electrophoresis using agarose gel at concentration 2%. Results of viral DNA amplification were positive, which revealed as band in 331 bp the results showed that ill yearling pigeons recording high infectivity rate (66.7%)compare with healthy yearling pigeons and adult once, the bursa of Fabricius samples of dead yearling pigeons recorded high prevalence (36.58%) when compare with liver and spleen samples, DNA of pigeon circovirus high detected (60.71%) in bursa of Fabricius of dead in shell pigeon embryo. inconclusion pigeon circovirus affected the racing pigeon in Mosul, Iraq.

Propagation of Salmonella enterica serovar typhimurium in embryonated chicken egg

Ammar Al-AAlim; Sumaya Aldabbagh; Haiffa Hussen Alkazaly; Mozahim Al-Attar

Iraqi Journal of Veterinary Sciences, 2019, Volume 33, Issue 2, Pages 81-86
DOI: 10.33899/ijvs.2019.163171

The study included the injection of Salmonella enterica serovar typhimuriumisolated from starlings bird in embryonated chicken eggs. The eggs were divided into eight groups, each group contain 6 eggs. The G1 and G2 groups were injected with the sterile normal saline solution in the choriaollantoic membrane (CAM) and yolk sac as negative control. The group G3 and G4 injected by bacterial suspension at a concentration of 104 cfu/ml in the CAM and yolk sac while the G5 and G6 injected with bacterial concentration 106 cfu/ml in the CAM and yolk sac respectively. Finally, the G7 and G8 groups were injected with 108 cfu/ml of bacterial suspension in the CAM and yolk sac respectively. The results showed that the highest percentage of death in eggs embryos was 100% in the sixth group after 96 hours of injection. There was also a significant increase in the number of bacteria in correlation with time of incubation. The highest rate of bacterial isolate was 8,19log10, 8,26log10 after 96 and 144 hours in the sixth group, while the highest number of bacterial isolates was 7.04log10 and 6.31log10 in the third and fourth groups after 48 and 96 hours of injection respectively. The results of the statistical analysis showed a significant difference in the number of bacterial isolate after 24 hours of injection in both concentrations compared to other incubation times. A significantrelationship was also found between the amount of the dose used and the bacterial disease. this study concluded that Salmonella enterica serovar typhimurium isolated from starlings can cause pathological changes and effect on hatchery percentage in embryonated chicken eggs.

Immunosuppressive effect of Marek's disease virus in broiler

Mozahim Yasen Al-Attar

Iraqi Journal of Veterinary Sciences, 2005, Volume 19, Issue 1, Pages 1-6
DOI: 10.33899/ijvs.2005.37262

تمت دراسة التأثیر المناعی المثبط للاصابة التجریبیة بفایروس مرض میرک فی فروج اللحم على مستوى المناعة المتکونة عند تلقیح حیوانات التجربة بلقاح مرض نیوکاسل وقد تم الکشف عن مستوى الأضداد المناعیة باستخدام اختبار تثبیط التلازن الدموی، حیث أظهرت نتائج هذا الاختبار انخفاض معیار الأضداد المتکونة ضد مرض نیوکاسل فی المجموعة الأولى المصابة تجریبیاً بفیروس مرض میرک والتی جرى تلقیحها بلقاح نیوکاسل بالمقارنة مع المجموعة الثانیة والتی اعطیت لقاح نیوکاسل فقط دون احداث الاصابة التجریبیة بفیروس مرض میرک، کما استخدم اختبار التحدی للتأکد من مستوى الحمایة التی أحدثها لقاح نیوکاسل حیث تبین أن هذا اللقاح أعطى حمایة بنسبة 50% فی المجموعة الأولى بینما کانت تلک النسبة 90% فی المجموعة الثانیة عند استخدام فیروس مرض نیوکاسل الضاری کجرعة التحدی لکلتا المجموعتین. وهذا یدل على أن الإصابة بفیروس مرض میرک فی الدواجن له تأثیر سلبی على مستوى المناعة التی یحدثها لقاح مرض نیوکاسل فی الدواجن.