Author : J. Hassain, Kh.


Detection of anti brucella antibodies in lambs and goat kids using rose bengal test and indirect ELISA in Gugjeli - Ninavah province, Iraq

Kh. J. Hassain; S. D. Hassan; B. A. Mohmmed; S. A. Esmaeel

Iraqi Journal of Veterinary Sciences, 2010, Volume 24, Issue 1, Pages 23-26
DOI: 10.33899/ijvs.2010.5566

The object of the study was to detection of anti-brucella antibodies in lambs and goat kids by using Rose Bengal and indirect ELISA tests. Sera samples were collected from four flocks, including 135 samples (95 from lambs and 40 from goat kids). The sera examined by rose Bengal test showed negative result in all of flocks, in percentage was zero %. While the percentage of anti-brucella antibodies using indirect ELISA test in female and male lambs was (29.34 %, 10 %) respectively, with total percentage 25.3% and in female and male kids was (55 %, 0.0 %) respectively, with total percentage 27. 5%. The results showed incompatibility between rose Bengal test and indirect ELISA test with kappa value. The study concluded that indirect ELISA test can be considerable as a screening and diagnostic test instead of rose Bengal test for detection of anti-brucella antibodies in lambs and kids.