Author : S. Ahmed, N.

Distribution of alkaline and acid phosphatases in the duodenal wall of native black goats by using different fixatives

N. S. Ahmed

Iraqi Journal of Veterinary Sciences, 2010, Volume 24, Issue 2, Pages 169-176
DOI: 10.33899/ijvs.2010.5595

Ten duodeni of adult goat were fixed in chilled acetone, 80% ethyl alcohol, alcohol-formalin solution, alcohol bouins solution and buffered neutral formalin solution. The distribution of alkaline and acid phosphatases noticed in absorptive and goblet cells that lining the duodenal mucosa of black goat, but different in their intensity and distribution according to different fixatives. The distribution of alkaline phosphatase in absorptive columnar cells that lining intestinal glands was more intense than other cells, whereas the concentration of acid phosphatase was more intense in goblet cells than other cells in the mucosa of goat duodenum specially in samples fixed in chilled acetone and ethyl alcohol 80%. The study revealed that the samples were fixed with chilled acetone gave highest reaction for alkaline and acid phosphatases than other fixative samples. No reaction for alkaline and acid phosphatases included some absorptive cells lining villi, all cells lining the lower parts of intestinal glands, paneth cells and submucosal glands in different fixatives, except submucosal glands revealed positive reaction for acid phosphatase in samples fixed in chilled acetone and 80% ethyl alcohol, paneth cells reveal positive reaction for the same enzyme in samples fixed in 80% ethyl alcohol in all examined areas of the duodenum wall of the native black goat.