Author : D. Hassan, S.

Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city

S. A. Asmaeel; Kh. J. Hussain; S. H. Arslan; S. D. Hassan

Iraqi Journal of Veterinary Sciences, 2010, Volume 24, Issue 2, Pages 89-92
DOI: 10.33899/ijvs.2010.5608

The aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas in Mosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis in sheep. The study included examination of 228 serum samples representing 1906 sheep distributed in ten flocks.The results of Rose Bengal test showed that total seroprevalence of brucellosis was (8.7%), being highest in Talkaf (40%), while no seroprevalence of Brucellosis was recorded in Al-Rashedia, Bazwaya and Al-Chaban areas. Using indirect ELISA test the total seroprevalence was (23.6%) and the highest percentage (100%) was in Al-Namrood area, but lowest (5.2%) in Bazwaya area. The results showed that the compatibility between the two tests was (0.380) on Kappa value which indicated that the indirect ELISA test is more sensitive when compared with Rose Bengal test in detection of antibodies of Brucellosis in serum sheep.